摘要
目的观察厄贝沙坦能否减轻糖尿病肾病(DN)大鼠的肾损伤。方法将大鼠随机分为对照组、DN模型组、厄贝沙坦治疗组。于22周测体质量、随机血糖、24 h尿微量白蛋白、血肌酐、尿素氮,观察肾脏形态改变。应用荧光定量PCR检测miR-192 mRNA在大鼠肾组织中的表达;蛋白免疫印迹法、免疫组织化学法检测肾组织TGF-β1、Zeb1、collagenⅠ蛋白的表达。结果与对照组比较,DN组大鼠体质量、随机血糖、血肌酐、尿素氮和24 h尿微量白蛋白显著增高(P<0.05);与DN组比较,厄贝沙坦治疗组大鼠体质量、随机血糖、血肌酐、尿素氮和24 h尿微量白蛋白显著降低(P<0.05)。厄贝沙坦治疗可以改善DN大鼠的一般情况和肾脏病理损害。与对照组相比,DN组miR-192 mRNA在DN大鼠肾脏的表达下调(P<0.05);与DN组相比,厄贝沙坦组miR-192 mRNA表达上调(P<0.05);与对照组大鼠相比,DN组肾组织的TGF-β1、ZEB1和collagenⅠ蛋白表达量明显增加(P<0.05);与DN组相比较,厄贝沙坦治疗可以减少TGF-β1、ZEB1和collagenⅠ蛋白的表达(P<0.05)。结论厄贝沙坦可减轻糖尿病肾病大鼠的肾损伤。
Objective To observe the effect of Irbesartan on renal injury in diabetic nephropathy(DN)rats.MethodsThe rats were randomly divided into control group,DN model group and Irbesartan treatment group.At the age of 22 weeks,body weight,random blood sugar,serum creatinine,urea nitrogen,and 24-hour urinary microalbumin were measured.The morphological changes of kidneys were observed.The expression of microRNA-192 in rat kidney was detected by fluorescence quantitative PCR,the expression of TGF-beta 1,Zeb1 and collagenⅠin rat kidney were detected by Western blot and immuno-histochemistry.Results Compared with the control group,the body weight,random blood sugar,serum creatinine,urea nitrogen,and 24-hour urinary micro-albumin of the model group rats increased significantly(P<0.05);Compared with the DN group,the body weight,random blood sugar,serum creatinine,urea nitrogen,and 24-hour urinary micro-albumin of the Irbesartan treatment group rats decreased significantly(P<0.05).Irbesartan treatment can improve the general condition and renal pathological damage of DN rats.Compared with the normal control group,the expression of microRNA-192 in the kidney of DN rats in the DN model group was down-regulated(P<0.05),while that in the Irbesartan group was up-regulated(P<0.05).WB and immuno-histochemical results showed that the expression of TGF-beta 1,ZEB1 and collagenⅠprotein in kidney tissue of DN modelgroup was significantly increased as compared with that of normal group(P<0.05);Compared with DN group,Irbesartan treatment reduced expression of TGF-beta 1,ZEB1 and collagenⅠprotein,with statistical significance(P<0.05).Conclusions Irbesartan attenuates renal damage in diabetic nephropathy rats.
作者
张娅
黄文辉
侯智敏
刘菊香
刘静
ZHANG Ya;HUANG Wen-hui;HOU Zhi-min;LIU Ju-xiang;LIU Jing(Gansu Provincial People's Hospital, Lanzhou 730000;Department of Clinical Medicine,Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China)
出处
《基础医学与临床》
CSCD
2020年第4期506-511,共6页
Basic and Clinical Medicine
基金
甘肃省人民医院院内科研基金(17GSSY4-2)。
