摘要
目的通过检测与创面愈合进程相关的组织基因表达,探讨采用负压创面治疗(nagetive pressure wound therapy,NPWT),促进糖尿病足溃疡(diabetic foot ulcer, DFU)愈合的作用机制。方法将纳入的50例DFU患者随机分成NPWT组(25例)和对照组(25例)。对照组接受局部湿敷治疗。两组患者治疗前后10 d均取创面肉芽行组织活检,利用RT-PCR检测转移生长因子-β1(transforminggrowth factor beta 1, TGF-β1)、血管内皮生长因子(vascular endothelial growth factor, VEGF)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白介素-1β(interleukin-1β, IL-1β)、基质金属蛋白酶-1(matrix metalloproteinase-1, MMP-1)、基质金属蛋白酶-9(matrixmetalloproteinase-9, MMP-9)和基质金属蛋白酶抑制剂-1(tissue inhibitor of metalloproteinase-1, TIMP-1)的mRNA表达,并进行对比分析。结果治疗后10 d,NPWT组患者创面肉芽组织中VEGF、TGF-β1和TIMP-1 mRNA的表达较治疗前显著增加,IL-1β、TNF-α、MMP-1和MMP-9 mRNA的表达显著下降(P<0.05);而对照组上述各项指标的mRNA表达,治疗前后均无显著变化(P>0.05)。结论 NPWT可能通过影响生长因子、炎症细胞因子和基质金属蛋白酶的表达来促进DFU愈合。
Objective To investigate the mechanism of negative pressure wound therapy(NPWT) in the promotion of wound healing of diabetic foot ulcer(DFU) by detecting the expression of tissue gene related to the process of wound healing. Methods Fifty patients with DFU were randomly divided into NPWT group(25 patients) and control group(25 patients). The local moist therapy was given to the control group. Before and 10 days after treatment, the biopsies of wound granulation tissues were performed in both groups. The m RNA expression of transforming growth factor beta 1(TGF-β1), vascular endothelial growth factor(VEGF), tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β), matrix metalloproteinase-1(MMP-1), matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) were detected by RT-PCR. The results were compared and analyzed. Results At 10 days after treatment, the m RNA expression of VEGF, TGF-β1 and TIMP-1 in wound granulation tissues of NPWT group was significantly increased, while the IL-1β,TNF-α, MMP-1 and MMP-9 significantly decreased(P<0.05). In the control group, there were no significant differences in all above indexes before and after treatment(P>0.05). Conclusion This investigation suggested that NPWT may promote wound healing of DFU by influencing the expression of growth factors, inflammatory cytokines and matrix metalloproteinases.
作者
芮昊
黄泽林
杨俊涛
谢莎莎
封海岗
程飚
左俊
RUI Hao;HUANG Ze-lin;YANG Jun-tao;XIE Sha-sha;FENG Hai-gang;CHENG Biao;ZUO Jun(The Ye star Medical Aesthetic Hospital of Heilongjiang,Harbin 150010,China)
出处
《中国美容整形外科杂志》
CAS
2020年第3期182-185,I0011,共5页
Chinese Journal of Aesthetic and Plastic Surgery
基金
广东省科技计划项目(2015B020233012).
关键词
负压创面治疗
糖尿病足溃疡
生长因子
炎症细胞因子
基质金属蛋白酶
Negative pressure wound therapy
Diabetic foot ulcer(DFU)
Growth factors
Inflammatory cytokines
Matrix metalloproteinases
