摘要
目的:基于系统药理学,探讨复方血藤颗粒的药效作用以及配伍机制。方法:通过中药系统药理数据库和分析平台(Traditional Chinese Medicines Systems Pharmacology Platform,TCMSP)数据库检索获得复方血藤颗粒中3个药物的化学成分、作用靶点,构建“化合物-靶点”网络,进行网络拓扑学分析,确定网络关键节点或关键模块,利用DVAID数据库进行GO富集分析、基于京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)的通路富集分析,分析其基因集合的主要功能,多层次、多角度探讨复方血藤颗粒的作用机制。结果:“化合物-靶点”网络包含30种活性成分,相应靶标94个。GO富集主要在细胞增殖的正调控、一氧化氮生物合成过程的正调控等方面,KEGG通路主要富集磷脂酰肌醇3-激酶(Phosphatidylinositol-3-Kinases,PI3K)/蛋白质丝氨酸苏氨酸激酶(Protein-serine-threonine Kinase,AKT)信号通路、缺氧诱导因子-1(Hypoxia-inducible Factor,HIF-1)信号通路、神经活性配体-受体相互作用通路等。结论:本研究结果初步验证了复方血藤颗粒的基本药理作用及其制,并为进一步深入揭示其作用机制奠定了良好基础。
Objective:To explore the pharmacodynamic effects and compatibility mechanism of the Fufang Xueteng granules(复方血藤颗粒)based on systemic pharmacology.Methods:The chemical constituents and effect target of three drugs in the Fufang Xueteng granules were retrieved by TCMSP database.The“compound-target”network was constructed,network to pology-analysis was performed;and the key nodes or key modules of the network were determined.The main function so fits gene collection was analyzed,and the mechanism faction of the Fufang Xueteng granules at multiple levels and multiple angles was explored by GO enrichment analysis and the enrichment analysis of KEGG used the DVAID database.Results:The“compound-target”network contains 30 active ingredients with 94 targets.GO enrich mentis mainly in the positive regulation of cell proliferation and the positive regulation of NO biosynthesis.The KEGG pathway is mainly enriched in PI3K/AKT signaling pathway,HIF-1 signaling pathway,neuroactive ligand-receptor interaction pathway,etc.Conclusion:The basic pharmacological effects and system of the Fufang Xueteng granules were preliminarily verified,and laid a good foundation for further revealing its mechanism faction.
出处
《中医临床研究》
2020年第1期1-7,共7页
Clinical Journal Of Chinese Medicine
基金
广西中医药民族医药传承创新专项(GZZJ14-04)。
关键词
复方血藤颗粒
系统药理学
信号转导通路
The Fufang Xueteng granule
Systematic Pharmacology
Signal Transduction Pathway
