摘要
目的探讨油酰乙醇胺(OEA)对糖尿病小鼠脑内炎性和神经营养因子的影响。方法采用高脂高糖加STZ链脲佐菌素(STZ)诱导T2DM小鼠模型,OEA[15、30、60 mg/(kg·d)]连续腹腔注射给药6周,通过Real time-PCR及Western blot检测海马内白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、突触成长相关蛋白-43(GAP-43)、突触素(SYN)、BDNF和NT-3的mRNA和蛋白表达水平。结果糖尿病模型小鼠海马内炎症因子IL-6、IL-1β和TNFα的表达水平下降,并显著上调海马内GAP-43、SYN、BDNF和NT3 mRNA表达水平。结论OEA具有降低糖尿病后海马内炎症,增加海马内突触相关因子和神经营养因子的作用,从而发挥神经保护作用。
Objective To investigate the effects of oleoylethanolamine(OEA)on inflammatory and neurotrophic factors in the brain of diabetic mice.Methods T2DM mouse model was induced by high-fat,high-glucose and STZ streptozotocin(STZ),OEA[15,30,60 mg/(kg·d)]was administered by intraperitoneal injection for 6 weeks,through Real time-PCR and Western blot analysis of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),synaptic growth-related protein-43(GAP-43),mRNA and protein expression levels of synaptophysin(SYN),BDNF and NT-3.Results The expression levels of inflammatory cytokines IL-6,IL-1βand TNFαin hippocampus of diabetic mice decreased,and the expression levels of GAP-43,SYN,BDNF and NT3 mRNA in hippocampus were significantly up-regulated.Conclusion OEA can reduce the inflammation in the hippocampus after diabetes,increase the synaptic related factors and neurotrophic factors in the hippocampus,and thus exert neuroprotective effects.
作者
刘金凤
许东伟
王幼娟
杨武双
LIU Jin-feng;XU Dong-wei;WANG You-juan;YANG Wu-shuang(Department of Pharmacy,Xiamen Hospital of Traditional Chinese Medicine,Xiamen,Fujian Province,361009 China;Department of Neurosurgery,Xiamen Hospital of Traditional Chinese Medicine,Xiamen,Fujian Province,361009 China)
出处
《糖尿病新世界》
2020年第1期15-17,共3页
Diabetes New World Magazine
基金
厦门市科技局项目(35020Z20174029)。
关键词
油酰乙醇胺
糖尿病脑损害
海马
神经保护
Oleoylethanolamine
Diabetic brain damage
Hippocampus
Neuroprotection
