摘要
目的从家蝇幼虫肠道细菌中筛选拮抗球孢白僵菌的细菌。方法家蝇幼虫肠道细菌的分离鉴定采用传统分离培养法,并通过细菌的菌落形态和16S rDNA序列进行鉴定;通过孢子萌发实验,筛选抑制球孢白僵菌孢子萌发的家蝇肠道细菌;通过平板对峙实验,筛选抑制球孢白僵菌菌丝生长的家蝇肠道细菌。结果采用传统分离培养法,在家蝇幼虫肠道中分离到13种细菌。通过孢子萌发实验表明,仅铜绿假单胞菌对球孢白僵菌孢子萌发有显著抑制作用,铜绿假单胞菌处理后,球孢白僵菌的孢子萌发率约为17%,其余细菌与对照组的孢子萌发率均在90%以上。通过平板对峙实验表明,铜绿假单胞菌可显著抑制球孢白僵菌菌丝的生长,在平板出现抑菌圈的平均直径可达30 mm左右,其他家蝇幼虫肠道细菌对球孢白僵菌菌丝生长均无抑制作用。结论家蝇幼虫肠道中的铜绿假单胞菌可抑制球孢白僵菌的孢子萌发和菌丝生长,可能是家蝇幼虫对球孢白僵菌免疫的原因之一。
Objective To screen for bacteria against Beauveria bassiana from the intestinal bacteria of Musca domestica larvae.Methods The traditional isolation and culture methods were used to isolate intestinal bacteria from M.domestica larvae,and the bacteria were identified by bacterial colony morphology and 16 S rDNA sequencing.The spore germination test was used to screen for the intestinal bacteria of M.domestica inhibiting the spore germination of B.bassiana.The plate confrontation test was used to screen for the intestinal bacteria of M.domestica inhibiting the mycelial growth of B.bassiana.Results Thirteen species of bacteria were isolated from the intestines of M.domestica larvae by the traditional isolation and culture methods.The spore germination test showed that only Pseudomonas aeruginosa significantly inhibited the spore germination of B.bassiana,with a germination rate of 17%,while the spore germination rates for other bacteria and control group were above 90%.The plate confrontation test showed that P.aeruginosa significantly inhibited the mycelial growth of B.bassiana,and that the mean diameter of the inhibitory zone on the plate was about 30 mm,while other intestinal bacteria from M.domestica larvae did not inhibit the mycelial growth of B.bassiana.Conclusion Pseudomonas aeruginosa in the intestines of M.domestica larvae can inhibit the spore germination and mycelial growth of B.bassiana,and this may be one reason why M.domestica larvae are immune to B.bassiana.
作者
万晴
黄振东
薛志静
乐倩倩
张瑞玲
张忠
WAN Qing;HUANG Zhen-dong;XUE Zhi-jing;LE Qian-qian;ZHANG Rui-ling;ZHANG Zhong(College of Basic Medical Science of Shandong First Medical University,Tai′an 271016,Shandong Province,China;Shandong Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases)
出处
《中国媒介生物学及控制杂志》
CAS
2020年第1期36-40,共5页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金(81572028,81401693,81271874)。
关键词
家蝇幼虫
球孢白僵菌
铜绿假单胞菌
抑菌实验
Musca domesticalarvae
Beauveria bassiana
Pseudomonas aeruginosa
Antifungal test
