摘要
【目的】进行离体培养关键因子优化,以满足火焰南天竹Nandina domestica‘Firepower’的规模化生产。【方法】以火焰南天竹茎段为外植体,通过对影响不定芽诱导、增殖生长和生根的基本培养基、6-苄基腺嘌呤(6-BA)和萘乙酸(NAA)不同质量浓度配比、白砂糖质量浓度及pH值等关键因子的研究,优化火焰南天竹茎段离体培养再生体系。【结果】WPM+0.50 mg·L^-16-BA+0.10 mg·L^-1 NAA为火焰南天竹不定芽诱导的理想培养基,不定芽诱导率为93.8%,且生长健壮;WPM+0.75 mg·L^-16-BA+0.10 mg·L^-1 NAA为不定芽增殖培养基,增殖倍数3.3,新芽鲜绿色、生长良好;1/2WPM+0.50 mg·L^-1 NAA+白砂糖30.0 g·L^-1(pH 6.0)为生根培养基,生根率达95.0%。【结论】本研究建立的火焰南天竹的离体培养再生体系可为其大规模生产提供技术支撑。
[Objective]To realize the large-scale production of Nandina domestica‘Firepower’,this research is focused on the optimization of the regeneration system of the stem segments of‘Firepower’in vitro.[Method]With stem segments of‘Firepower’as the explant,a study is conducted of the key factors such as the basic medium,6-BA and NAA concentration,sugar concentration and pH that affect the adventitious bud induction,proliferation and rooting.[Result](1)The ideal medium for adventitious bud induction was WPM+0.50 mg·L^-16-BA+0.10 mg·L^-1 NAA,with an induction rate of adventitious bud of 93.8%,and a robust growth rate;(2)The medium for adventitious bud multiplication was WPM+0.75 mg·L^-16-BA+0.10 mg·L^-1 NAA,with a proliferation cofficient of 3.3 and favorable growth of the new bud;(3)The rooting medium was 1/2 WPM+0.50 mg·L^-1 NAA+white sugar 30.0 g·L^-1(pH 6.0),with a rooting rate of 95.0%.[Conclusion]The regeneration system of the stem segments of‘Firepower’in vitrothat has been optimized in the current study will provide reliable technical support for large-scale production of‘Firepower’.
作者
叶雯
袁超群
秦伟
王振涛
朱玉球
YE Wen;YUAN Chaoqun;QIN Wei;WANG Zhentao;ZHU Yuqiu(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2020年第2期391-396,共6页
Journal of Zhejiang A&F University
基金
嘉兴市科技计划项目(SQ2017000726)。
关键词
火焰南天竹
组织培养
基本培养基
植物生长调节剂
Nandina domestica‘Firepower’
tissue culture
basic medium
plant growth regulator
