摘要
为研究裸大麦的遗传多样性,利用筛选得到的16条ISSR引物对收集的国内外102份裸大麦材料和1份皮大麦材料进行了遗传多样性分析,并分析了裸大麦地理分布与遗传多样性的关系。结果表明,16条ISSR引物共扩增出133条条带,大小在100~2000bp。不同引物扩增的条带数在3?15条,平均每条引物扩增出8.31条,多态性条带为113条,多态性的平均百分率为85.1%,103份材料的遗传相似系数(GS)在0.2817~6,提示裸大麦的遗传多样性较高。利用NTsys 2.1软件进行聚类分析,103份材料分为四大类,第一大类只分布在中国青海,第二大类分布在亚洲、欧洲、北美洲和非洲,第三大类分布在亚洲、北美洲和南美洲,第四大类仅分布在亚洲和北美洲,表明亚洲,尤其中国地区具有较高的遗传多样性。裸大麦的遗传多样性与其地理来源的相关性,为进一步研究裸大麦尤其是青稞起源提供了参考。
In order to study the genetic diversity of naked barley,102 naked barley and 1 hulled barley collected at home and abroad were analyzed by using 16 ISSR primers,and the relationship between geographical distribution and genetic diversity of naked barley was also analyzed.The results showed that 133 bands were amplified by 16 ISSR primers,and the size was 100?2000 bp.The numbers of bands amplified by different primers ranged from 3 to 15,with an average of 8.31 per primer and the polymorphic bands was 113.The average percentage of polymorphisms was 85.1%.The genetic similarity coefficient(GS)of 103 materials was 0.2817?6,suggesting that the genetic diversity of naked barley was higher.The cluster analysis using NTsys 2.1 software showed that 103 materials were divided into four categories:the first group was only distributed in Qinghai Province,China,the second group was distributed in Asia,Europe,North America and Africa,the third group was distributed in Asia,North America and South America,and the fourth group was distributed in Asia and North America,indicating that in Asia,especially China area has higher genetic diversity.The correlation between genetic diversity and geographical origin of naked barley provides a reference for further study on the origin of naked barley,especially Tibetan naked barley.
作者
张超
才让卓玛
卡毛先
段瑞君
Zhang Chao;Cairang Zhuoma;Ka Maoxian;Duan Ruijun(College of Ecol-Environmental Engineering,Qinghai University,Xining,810016;Qinghai Provincial Key Laboratory of Hulless Barley Genetics and Breeding,Qinghai Academy of Agricultural and Forestry Sciences,Xining,810016)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第4期1193-1201,共9页
Molecular Plant Breeding
基金
国家自然科学基金(31560052)
青海省科技厅应用基础研究项目(2016-ZJ-772,2019-ZJ-7049)共同资助。
