摘要
为了降低霉菌在果蔬采后贮藏过程中的危害,本实验以桃吉尔霉(Gilbertella persicaria)为材料,研究了主动自发气调对桃吉尔霉生长的调控作用。实验分别设置80%(体积分数,下同)O2+20%CO2、90%O2+10%CO2、100%O2和自然空气(CK)4个处理组,将桃吉尔霉分别置于含上述比例气体的密封袋内,室温(20.0±0.5)℃培养,研究主动自发气调对菌体孢子量、电导率、孢子DNA释放量、活性氧防御酶及细胞壁降解酶活力的影响。结果表明:O2/CO2主动自发气调在O2、CO2协同作用下,对桃吉尔霉的生长有良好的控制效果,且随着CO2体积分数的增大,对菌体的伤害增大,80%O2+20%CO2处理对桃吉尔霉细胞产生较大损伤,抑制了孢子的生长。同时,主动自发气调处理降低了超氧化物歧化酶、过氧化物酶以及细胞壁降解酶活力,增大了孢子DNA的释放和孢子细胞膜渗透性。实验结果为降低果蔬贮藏期间霉菌致病性、自发气调抑菌提供了理论依据。
The objective of this study was to investigate the effect of active modified atmosphere(MA) on the growth of Gilbertella persicaria in order to reduce postharvest fungal diseases during the storage of fruits and vegetables. In this study, four treatments were set up: 80% O2 + 20% CO2, 90% O2 + 10% CO2, 100% O2 and natural air(control), and Gilbertella persicaria was sealed in each of the four gas atmospheres and cultured at(20.0 ± 0.5) ℃. The amount of spores, conductivity, DNA release of spores, enzyme activities associated with defense against reactive oxygen species(ROS), and cell wall degrading enzymes in Gilbertella persicaria were evaluated. The results showed that active MA was effective in controlling the growth of Gilbertella persicaria through the synergy between O2 and CO2. With the increase of CO2 content, greater damage was observed to Gilbertella persicaria. The MA of 80% O2 + 20% CO2 caused the greatest damage to this strain and inhibited the production of spores. Additionally, active MA reduced the activities of superoxide dismutase(SOD), peroxidase(POD) and cell wall degrading enzymes and increased DNA release of spores and membrane permeability. The results of this study provide a theoretical basis for reducing the pathogenicity of Gilbertella persicaria in fruits and vegetables during storage and for inhibiting it by active MA.
作者
刘莎莎
张玉笑
王亮
员丽苹
陈勇
张新华
郭衍银
LIU Shasha;ZHANG Yuxiao;WANG Liang;YUN Liping;CHEN Yong;ZHANG Xinhua;GUO Yanyin(School of Agricultural Engineering and Food Science,Shandong University of Technology,Zibo 255049,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第5期186-192,共7页
Food Science
基金
国家自然科学基金面上项目(31671900)
淄博市校城融合发展计划项目(2018ZBXC142)。
关键词
主动自发气调
桃吉尔霉
致病性
果蔬
保鲜
active modified atmosphere
Gilbertella persicaria
pathogenicity
fruits and vegetables
preservation
