摘要
目的研究5α-二氢睾酮(DHT)对雄性BALB/c小鼠Grave病模型发生发展及其脾脏淋巴细胞因子分泌的影响。方法6~8周龄雄性BALB/c小鼠按数字表法随机分为对照组、造模组、安慰剂组和DHT组4组。用腺病毒表达的人促甲状腺素受体抗体A-亚单位(human thyroid stimulating hormone receptor antibody A-subunit,Ad-TSHR289)免疫雄性BALB/c小鼠诱导建立Grave病模型,在免疫前1周DHT组皮下植入5mg DHT,安慰剂组植入安慰剂,观察DHT对小鼠甲状腺功能的影响;用流式细胞学方法检测小鼠的脾脏淋巴细胞分泌干扰素γ(IFNγ)、白细胞介素(IL)-4、IL-10、IL-9及IL-17的细胞百分比变化。结果造模组小鼠的总甲状腺素[(117.76±32.69)nmol/L比(33.08±12.61)nmol/L]与游离甲状腺素[(15.01±11.55)pmol/L比(3.55±1.88)pmol/L]明显高于对照组,P值均<0.0001;DHT组小鼠的甲状腺功能略低于安慰剂组,总甲状腺素(114.80±44.27)nmol/L比(123.17±77.73)nmol/L;游离甲状腺素(13.48±6.01)pmol/L比(14.19±12.65)pmol/L,但差异无统计学意义(P值均>0.05)。DHT组BALB/c小鼠脾脏分泌IL-10的2型辅助性T细胞(Th2)百分比明显高于安慰剂组[(7.11±3.29)%比(3.51±1.36)%,P<0.05];DHT组小鼠脾脏分泌IFNγ、IL-4、IL-9及IL-17的细胞百分比与安慰剂组差异无统计学意义(P>0.05)。结论DHT降低雄性BALB/c小鼠Grave病模型甲状腺功能的作用不甚显著。DHT可能通过上调细胞因子IL-10而发挥对雄性BALB/c小鼠Grave病模型的免疫调节作用。
Objective To study the influences of dihydrotestosterone(DHT)on the development of experimental autoimmune Graves disease(EAGD),and to observe the effect of DHT on cytokines in male BALB/c mice model.Methods Male BALB/c mice aged 6-8 weeks were divided into 4 groups using random number table:(1)control group;(2)EAGD group;(3)placebo group;(4)DHT group.EAGD mice were induced with an adenovirus expressing the human thyroid stimulating hormone receptor antibody A-subunit(Ad-TSHR289).DHT(5mg)or a matching placebo were implanted one week before the first immunization.Thyroid hormones were detected with radioimmunoassay kit..Cytokines[such as interferonγ(IFNγ),interleukin(IL)-4,IL-10,IL-9,and IL-17]producing cells from the spleen were detected using flow cytometry.Results As expected Ad-TSHR289 treatment increased total thyroxine[EAGD group vs.control group:(117.76±32.69)nmol/L vs.(33.08±12.61)nmol/L,P<0.0001]and free thyroxine[EAGD group vs.control group:(15.01±11.55)pmol/L vs.(3.55±1.88)pmol/L,P<0.0001].Treatment of DHT slightly lowered thyroid hormones[DHT group vs.placebo group:total thyroxine(114.80±44.27)nmol/L vs.(123.17±77.73)nmol/L;free thyroxine(13.48±6.01)pmol/L vs.(14.19±12.65)pmol/L],without significant difference(all P>0.05)].However,the percentage of IL-10,but not IFNγ,IL-4,IL-9 and IL-17,secreted spleen cells increased in DHT group than in the placebo group[(7.11±3.29)%vs.(3.51±1.36)%,P<0.05].Conclusion The effects of DHT on thyroid hormone are mild.It might play an immunomodulatory role in the male mouse Graves disease model by up-regulating the cytokine IL-10.
作者
刘莲叶
施秉银
伍丽萍
高爱博
Liu Lianye;Shi Bingyin;Wu Liping;Gao Aibo(Department of Endocrinology,The First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China;Department of Endocrinology,Weinan Central Hospital,Weinan,Shaanxi 714000,China)
出处
《中华内科杂志》
CAS
CSCD
北大核心
2020年第1期47-51,共5页
Chinese Journal of Internal Medicine
基金
国家自然科学基金(30972701)。
