摘要
构建了一种适配体修饰的Hg^2+传感器,以氧化铟锡(ITO)导电玻璃为基底电极,纳米TiO2为光电活性材料,通过金纳米星固定特异性识别单元巯基修饰适配体。当适配体特异性识别Hg^2+时,适配体富碱基T序列与Hg^2+结合形成折叠的发卡结构,传感器界面发生改变从而实现Hg^2+定量分析。采用时间-电流法、交流阻抗法等电化学方法对所构建的传感器检测性能进行研究。实验结果表明,在优化实验条件下,该方法用于检测Hg^2+的线性范围为1.0×10^-9~5.0×10^-7 mol/L,检测限为3.1×10^-10 mol/L。该传感器制备过程简单,具有较高的检测灵敏度,较高的稳定性和可重复性。
A photoelectrochemical method was developed for Hg^2+ detection using TiO2 nanoparticles as photoactive material,Au nanostars as immobilization substrate and-SH modified aptamer as recognition element.In the presence of Hg^2+,DNA aptamer could fold into a hairpin structure with"T-Hg^2+-T"recognition for quantitative analysis of Hg^2+.The performance of aptasensor was studied by electrochemical methods such as current-time curve and electrochemical impedance spectroscopy.Under optimal conditions,the aptasensor showed linear range for Hg^2+ detection from 1.0×10^-9 to 5.0×10^-7 mol/L and a detection limit of 3.1×10^-10 mol/L(3σ).The aptamer sensor is easy in preparation,highly sensitive,stable and repeatable.
作者
庄玉娇
巫仙群
王宇蕊
文勉
罗贵铃
孙伟
牛燕燕
ZHUANG Yujiao;WU Xianqun;WANG Yurui;WEN Mian;LUO Guiling;SUN Wei;NIU Yanyan(Key Laboratory of Functional Materials and Photoelectrochemistry of Haikou,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158)
出处
《分析科学学报》
CAS
CSCD
北大核心
2020年第1期116-120,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.1665007)
海南省自然科学基金(No.219QN207)
海南师范大学大学生创新项目(No.cxcyxj2018020)
海南省水环境污染治理与资源化重点实验室开放基金(2019-003)
