摘要
目的制备一种新型磁热相变型纳米粒造影剂(PFH-HIONS),研究其在体外增强光声成像、磁共振及磁热相变后增强超声显影的能力。方法先采用一锅溶剂热法制备超顺磁性纳米空心铁球(HIONS),再采用真空吸附法将相变材料液态氟碳全氟己烷(PFH)包载入空心铁球得到PFH-HIONS,对纳米粒进行表征后,分别在体外进行光声、磁共振及磁加热相变后超声显影,用软件分析显影强度,比较显影结果。结果成功制备出一种包载PFH的PFH-HIONS,粒径均匀,平均粒径约(537.3±24.8)nm。PFH-HIONS可增强光声成像和磁共振体外显影。在交变磁场内,其能显著加热并促进PFH相变产生微气泡,从而增强超声显影,并且随着浓度的增加,显影强度增强,不同浓度间显影强度差异均有统计学意义(P<0.05)。结论PFH-HIONS能增强超声、光声、磁共振多种模式显影,并且具有较好的磁加热性能,为分子影像学基础上的诊治一体化提供了新型、高效的研究平台,具有较好的应用前景。
Objective To prepare a novel magnetic heating phasetransition nanoparticle contrast agent(PFH-HIONS),and to study its performance on enhancing photoacoustic imaging,magnetic resonance imaging and ultrasound imaging after phasetransition by magnetic-thermo in vitro.Methods Firstly,the superparamagnetic nano hollow iron spheres(HIONS)were prepared by a one-pot solvothermal method,and then the phasetransition liquid perfluorocarbon(PFH)was loaded on the HIONS by vacuum adsorption to obtain PFH-HIONS.After characterization of the nanoparticles,photoacoustic imaging,magnetic resonance imaging and ultrasound imaging after phasetransition with magnetic-thermo were performed in vitro,and the results were analyzed by a software.Results PFH-HIONS was successfully prepared with uniform particle size of(537.3±24.8)nm.PFH-HIONS could apparently enhance photoacoustic imaging and magnetic resonance imaging in vitro.In an alternating magnetic field,it could significantly increase the temperature,which promotes phasetransion of the PFH to produce microbubbles,thereby enhancing ultrasound imaging.Furthermore,as the concentration increased,the imaging intensity was enhanced,and the differences in imaging intensity between different concentration groups were statistically significant(P<0.05).Conclusions The PFH-HIONS can enhance the multimodal imaging including ultrasound,photoacoustic and magnetic resonance,and it also has obvious magnetic heating performance.It provides a new and efficient research platform for theranostics based on molecular imaging,therefore it has promising application prospects.
作者
周洋
徐烽
刘莹
叶鸣
王志刚
赵宇心
Zhou Yang;Xu Feng;Liu Ying;Ye Ming;Wang Zhigang;Zhao Yuxin(Department of Ultrasound,the Second Affiliated Clinical Hospital of Chongqinng Medical University in Chengdu,the Third People′s Hospital of Chengdu City,Chengdu 610031,China;Department of Ultrasound,the Forestry Central Hospital of Aba Prefecture,Dujiangyan 611800,China;Institute of Ultrasound Imaging,Chongqing Medical University,Chongqing 400010,China)
出处
《中华超声影像学杂志》
CSCD
北大核心
2020年第1期77-82,共6页
Chinese Journal of Ultrasonography
基金
国家自然科学基金重点项目(31530026)
国家自然科学基金面上项目(81971636)
四川省卫计委科研项目(130417)
四川省医学科研课题计划项目(S17011)。
关键词
磁热
相变
造影剂
多模态
Magnetic heating
Phasetransition
Contrast agent
Multimodal
