摘要
Three nanostructured photosensitizers with aggregation-induced emission(AIE) characteristics based on2,3-bis(4?-(diphenylamino)-[1,1?-biphenyl]-4-yl) fumaronitrile(BDBF) were prepared for image-guided photodynamic therapy(PDT). BDBF was encapsulated with Pluronic F-127(F127) to form usual spherical nanoparticles(F127@BDBF NPs) with a red fluorescence emission and 9.8% fluorescence quantum yield(FQY). Moreover, BDBF self-assembled into nanorods(BDBF NRs) in water. Compared with F127@BDBF NPs, BDBF NRs exhibited stronger orange fluorescence with a higher FQY of 23.3% and similar singlet oxygen(1O2) generation capability. BDBF NRs were further modified with F127 to form BDBF@F127 NRs with the same 1O2 generation ability as BDBF NRs. The three nanostructures exhibited a higher 1O2 production capacity than BDBF molecule in dissolved state and favorable stability in an aqueous solution as well as under physiological condition. In vitro photocytotoxicity experiments indicated that the three nanostructures inhibited tumor cell proliferation effectively.Therefore, to construct eligible nanostructures with a high FQY and 1O2 generation ability, simple self-assembly can serve as a valuable method to prepare photosensitizers with enhanced PDT.
基于具有聚集诱导发光(AIE)性质的2,3-双(4’-(二苯基)-[1,1’-联苯]-4-基]富甲腈(BDBF)分子,制备了三种纳米结构并用于图像引导光动力学治疗(PDT).普兰尼克F127可包封BDBF形成常见的球形纳米粒子(F127@BDBF NPs),该纳米粒子可发射红色荧光,荧光量子效率(FQY)为9.8%.此外, BDBF也可在水中自组装成纳米棒(BDBF NRs).与F127@BDBF NPs相比, BDBF NRs呈现出较强的橙色荧光,具有较高的荧光量子产率(23.3%),以及基本相同的单线态氧(1O2)产生能力.利用F127对BDBF NRs进行进一步修饰可得到BDBF@F127 NRs,该纳米粒子仍然保持了棒状形貌和较好的1O2产生能力.同时,与溶解态的BDBF相比,三种纳米结构的单线态氧产生效率增强.这些纳米结构在水溶液和生理条件下具有良好的稳定性.细胞的光毒性实验表明,三种纳米结构均能有效抑制肿瘤细胞增殖.因此,通过简单的自组装方法制备高荧光量子效率和较强单线态氧产生能力的纳米结构可作为一种有效的途径来增强光动力.
作者
Wenkun Han
Song Zhang
Rong Deng
Yangyang Du
Jingyu Qian
Xiaohua Zheng
Bin Xu
Zhigang Xie
Fei Yan
Wenjing Tian
韩文坤;张松;邓荣;杜阳阳;钱靖宇;郑孝华;徐斌;谢志刚;闫飞;田文晶(State Key Laboratory of Supramolecular Structure and Materials,College of Chemistry,Jilin University,Changchun 130012,China;State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,International Joint Research Laboratory of Nano-Micro Architecture Chemistry(NMAC),International Research Center for Chemistry-Medicine Joint Innovation,College of Chemistry,Jilin University,Changchun 130012,China;State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)
基金
financially supported by the National Natural Science Foundation of China (21835001, 51773080, 21674041, 51573068, 21221063, and 81870117)
the Program for Changbaishan Scholars of Jilin Province, Jilin Province project (20160101305JC)
Jilin Province Science and Technology Development Plan (20190201252JC)
“Talents Cultivation Program” of Jilin University
