摘要
目的探讨腹泻型肠易激综合征(IBS)患者结肠灌洗液优势菌群的特征。方法选择2016年1月至2019年1月深圳市龙华区人民医院消化内科收治的腹泻型IBS患者46例(观察组)和排除IBS的腹痛或腹部不适患者46例(对照组)。于结肠镜检查时钳取降结肠黏膜组织2块,再收集所取黏膜组织处结肠灌洗液4 ml,提取总DNA,并以实时荧光定量聚合酶链反应法对10种优势菌进行检测(包括拟杆菌门、普氏杆菌、双歧杆菌属、梭菌属、肠致病性大肠埃希菌、害肺戴阿里斯特杆菌、厚壁菌门、普拉梭菌、沙门菌和乳杆菌属;结果取对数值)。采用Spearman法对结肠黏膜组织和结肠灌洗液的优势菌进行相关性分析。结果两组结肠黏膜组织和灌洗液优势菌群组成以及菌群变化基本一致,其中观察组结肠黏膜组织和结肠灌洗液中拟杆菌门、普氏杆菌、梭菌属菌群数及结肠灌洗液中普拉梭菌菌群数明显低于对照组(结肠黏膜组织:3.68±0.54比4.34±0.27、4.26±0.28比5.33±0.42、4.46±0.68比4.99±0.61;结肠灌洗液:3.26±0.61比4.09±0.51、3.10±1.42比4.86±1.03、5.24±0.54比5.95±0.51、2.24±1.83比3.24±1.46),差异有统计学意义(P<0.01或<0.05);而两组结肠黏膜组织和结肠灌洗液中其他菌群数比较差异无统计学意义(P>0.05)。Spearman相关性分析结果显示,结肠黏膜组织与结肠灌洗液中双歧杆菌属、厚壁菌门、普拉梭菌和乳杆菌属呈正相关(r=0.427、0.689、0.577和0.369,P=0.041、0.013、0.024和0.035),肠致病性大肠埃希菌和沙门菌呈负相关(r=-0.352和-0.649,P=0.026和0.013);而其他菌群无相关性(P>0.05)。结论腹泻型IBS患者结肠黏膜组织和结肠灌洗液中优势菌群构成基本一致,而且优势菌群均呈失调状态,其中结肠灌洗液中拟杆菌门、普氏杆菌、梭菌属、普拉梭菌的菌群数明显降低。
Objective To investigate the characteristics of dominant bacteria of colonic lavage fluid in patients with diarrhea-predominant irritable bowel syndrome(IBS).Methods Forty-six patients with diarrhea-predominant IBS(observation group)and 46 patients with abdominal pain or discomfort who was excluded IBS(control group)from January 2016 to January 2019 in Department of Gastroenterology,Shenzhen Longhua District People′s Hospital were selected.During colonoscopy,2 pieces of descending colonic mucosal tissues were taken by forceps,then 4 ml of colonic lavage fluid was collected from the mucosa tissues to extract total DNA,and 10 dominant bacteria were detected by real-time fluorescence quantitative polymerase chain reaction(including Bacteroides,Bacteriodes prevotella,Bifidobacterium,Clostridium,Enteropathogenic Escherichia coli,Dialister pneumosintes,Firmicutes,Faecalibacterium prausnitzii,Salmonella,Lactobacillus,and data were selected for logarithm).The correlation of dominant bacteria between colonic mucosal tissues and colonic lavage fluid was analyzed by Spearman correlation analysis.Results The composition and change of dominant bacteria of colonic mucosal tissues and colonic lavage fluid were basically the same in 2 groups.The numbers of Bacteroides,Bacteriodes prevotella,Clostridium in colonic mucosal tissues and colonic lavage fluid,and the numbers of Faecalibacterium prausnitzii in colonic lavage fluid of observation group were significantly lower than those in the control group(colonic mucosal tissues:3.68±0.54 vs.4.34±0.27,4.26±0.28 vs.5.33±0.42,4.46±0.68 vs.4.99±0.61;colonic lavage fluid:3.26±0.61 vs.4.09±0.51,3.10±1.42 vs.4.86±1.03,5.24±0.54 vs.5.95±0.51,2.24±1.83 vs.3.24±1.46),and there were statistical differences(P<0.01 or<0.05).There was no statistical differences in other dominant bacteria in colonic mucosal tissues and colonic lavage fluid between 2 groups(P>0.05).Spearman correlation analysis result showed that the Bifidobacterium,Firmicutes,Faecalibacterium prausnitzii,Lactobacil
作者
黄敏
邱嘉华
何小玲
Huang Min;Qiu Jiahua;He Xiaoling(Department of Gastroenterology,Shenzhen Longhua District People′s Hospital,Shenzhen 518100,China)
出处
《中国医师进修杂志》
2020年第1期53-56,共4页
Chinese Journal of Postgraduates of Medicine
关键词
肠易激综合征
腹泻
结肠灌洗液
肠道菌群
Irritable bowel syndrome
Diarrhea
Colonic lavage fluid
Intestinal microbiota
