摘要
为优选小花清风藤茎叶提取工艺的最佳提取条件,采用正交设计安排试验,选择料液比、提取温度、提取时间作为提取的因素,以四氯化碳损伤后的人正常肝细胞(LO2)细胞存活率为保肝活性指标,进行统计分析,确定最佳提取条件.优选出小花清风藤的最佳提取工艺条件为小花清风藤粉末10 g,加入200 mL水,80℃水浴加热1 h.该提取工艺设计合理,重复性强,可作为小花清风藤提取工艺.
To optimize extraction conditions of stem and leaf from Sabia parviflora Wall. ex Roxb, in this paper, orthogonal design was used and the ratio of material to liquid, extraction temperature and extraction time were selected as the extraction factors. The survival rate of normal human hepatic cell(LO2) damaged by carbon tetrachloride was considered as the index of hepatoprotective activity for statistical analysis. The results showed that the optimum extraction conditions were as follows: 10 g powder, 200 mL water and 80 ℃ water bath for 1 h. The extraction process is reasonable and repeatable, which would be applied in the extraction process of Sabia parviflora Wall. ex Roxb extraction.
作者
张学学
赵东晓
李建桥
卢顺光
胡建忠
李曼姝
刘朝霞
邹坤
ZHANG Xuexue;ZHAO Dongxiao;LI Jianqiao;LU Shunguang;HU Jianzhong;LI Manshu;LIU Zhaoxia;ZOU Kun(Hubei Key Laboratory of Natural Products Research and Development,College of Biological and Pharmaceutical Sciences,China Three Gorges University,Yichang,Hubei 443002,China;Soil and Water Conservation Centre,the Ministry of Water Resources,Beijing 100038,China)
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第1期65-69,73,共6页
Journal of Central China Normal University：Natural Sciences
基金
水利部水土保持植物开发管理中心合作项目(2017-zg-kj-4/SDHZ2017015
2018-zg-kj-03
SDHZ2019030)
国家自然科学基金青年科学项目(81803383)
关键词
小花清风藤
提取工艺
正交设计
保肝活性
Sabia parviflora Wall.ex Roxb
extracting technique
orthogonal design
liver protective activity
