摘要
目的比较手足口病患者咽拭、粪便样本中肠道病毒(EV)的检出率,为手足口病监测样本采集的选择提供依据。方法采集2013年间鞍山地区医疗机构临床诊断手足口病患者63例咽拭、粪便配对标本。采用Real-time PCR法进行柯萨奇病毒A组16型(Cox A16)、肠道病毒71型(EV 71)、其他肠道病毒型检测。结果 63例患者配对样本中,Cox A16、EV 71、肠道病毒通用型咽拭子阳性率分别为26.98%、1.59%和26.98%,粪便阳性率分别为22.22%、1.59%和31.75%。结果显示两种样本病毒核酸阳性率,差异无统计学意义(χ2=0.534,P>0.05)。两种样本检测结果相关性,差异有统计学意义(χ2=8.036,P<0.05)。咽拭样本和粪便配对样本核酸检测阳性率,差异无统计学意义(P>0.05)。结论咽拭样本和粪便样本核酸检测阳性率相同,可以采集咽拭样本代替粪便标本。
Objective To compare the detection rate of enterovirus(EV)in swallow swab and fecal samples of patients with hand-foot-mouth disease, and to provide evidence for the monitoring sample selection of hand-foot-mouth disease. Method In 2013, 63 patients with hand-foot-mouth disease clinically diagnosed by medical institutions in Anshan district were collected and paired samples of pharyngeal swabs and feces were collected.Cox A16, EV 71 and other enter virus types were detected by real-time PCR. Results Among the 63 pairs of specimens, the positive rates of Cox A16,EV 71 and other Etiology in throat swabs were 26.98%, 1.59% and 31.75% respectively, and in excrements were 22.22%, 1.59% and 31.75%respectively. The results showed that the positive rate of viral nucleic acid in the two samples was not statistically significant(χ2=0.534,P>0.05). The correlation between the two samples was statistically significant(χ2=8.036,P<0.05). There was no significant difference in the positive rate of nucleic acid detection between the throat swab and the fecal paired sample(P>0.05). Conclusion The positive rate of nucleic acid detection in throat swab and fecal samples is the same, and throat swab samples can be taken instead of fecal specimens.
作者
仲惟惟
尹晔
ZHONG Wei-wei;YIN Ye(Anshan Center for Disease Control and Prevention,Anshan,Liaoning,114011,China)
出处
《中国冶金工业医学杂志》
2020年第1期10-11,共2页
Chinese Medical Journal of Metallurgical industry
关键词
手足口病
病原学
肠道病毒
Hand-foot-mouth disease
Etiology
Enterovirus
