摘要
为阐明外源茉莉酸甲酯(MeJA)诱导的苹果(Malusdomestica)抗病分子机制,以生长30天的Gala组培苗为试材,用100μmol·L–1 MeJA处理叶片12小时,通过转录组测序,结合生物信息学分析鉴定出苹果叶片中受MeJA诱导表达的基因。结果表明,外源MeJA主要影响苹果叶片倍半萜类、三萜和类黄酮的生物合成,以及芸薹素(BR)信号转导途径间接诱导的抗病性;倍半萜类、三萜及类黄酮生物合成途径中的关键基因为MDP0000702120和MDP0000692178;MDP0000123379是联系芸薹素信号转导途径和植物-病原菌互作途径的关键调控基因。
To study the molecular mechanism of resistance induced by exogenous methyljasmonate(MeJA) in apple, tissue cultured seedlings of Gala grown for 30 days were used as materials. The leaves were treated with 100 μmol·L–1 MeJA for 12 h. RNA sequencing and bioinformatics analysis were conducted to identify differentially expressed genes induced by MeJA. Exogenous MeJA mainly affected the biosynthesis of sesquiterpenoids, triterpenoids and flavonoids in apple leaves and induced disease resistance by brassinolide signal transduction pathways indirectly. The key genes regulating sesquiterpenoid and triterpenoid biosynthesis and flavonoids biosynthesis are MDP0000702120 and MDP0000692178;MDP0000123379 was the key regulatory gene linked to the brassinosteroid signaling pathway and plant-pathogen interaction pathway.
作者
张娜
刘秀霞
陈学森
吴树敬
Zhang Na;Liu Xiuxia;Chen Xuesen;Wu Shujing(State Key Laboratory of Crop Biology,College of Horticultural Science and Engineering,Shandong Agricultural University,Tai’an 271018,China;College of Biology and Brewing Engineering,Taishan University,Tai’an 271018,China)
出处
《植物学报》
CAS
CSCD
北大核心
2019年第6期733-743,共11页
Chinese Bulletin of Botany
基金
国家重点研发计划(No.2018YFD1000307)
国家自然科学基金(No.31272132)
山东省泰山学者工程启动基金(No.tshw20120712)
关键词
茉莉素
抗病性
信号转导
转录组
苹果
jasmonates
disease resistance
signal transduction
transcriptome
apple
