摘要
目的探讨双酚A(bisphenol A,BPA)暴露与结直肠癌(colorectal cancer,CRC)肿瘤组织神经酰胺(ceramide,Cer)及血清肿瘤标志物的相关性。方法收集84例CRC患者的晨尿、CRC肿瘤组织,使用液相色谱-质谱联法(liquid chromatography mass spectrometer,LC-MS)检测尿液BPA浓度,尿BPA浓度用尿肌酐(creatinine,Cr)进行校正(BPAcr)。酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)法检测肿瘤组织Cer浓度,分析尿BPAcr与肿瘤组织Cer、血清肿瘤标志物之间的相关性。结果CRC肿瘤组织Cer与BPAcr呈正相关(r=0.784,P<0.001),回归分析显示肿瘤组织Cer与BPAcr的回归系数为0.218(95%CI:0.18~0.26),具有统计学意义(P<0.001);血清CEA阳性和阴性、CA125阳性和阴性、CA19-9阳性和阴性组之间CRC肿瘤组织Cer和尿BPAcr差异均有统计学意义(均有P<0.05),而AFP阳性和阴性组间肿瘤组织Cer和尿BPAcr差异无统计学意义(P=0.247)。血清CEA、CA125和CA19-9水平与尿BPAcr(r值分别为0.348,0.251,0.281,均有P<0.05)和CRC肿瘤组织Cer(r值分别为0.265,0.309,0.263,均有P<0.05)均呈正相关。结论BPA暴露可能会引起CRC肿瘤组织内Cer增加以及血清肿瘤标志物的异常,提示BPA暴露可能通过影响CRC肿瘤组织Cer代谢参与CRC的发生发展。
Objective The aim is to investigate the correlation between bisphenol A(BPA)exposure and tumor tissue ceramide(Cer)as well as serum tumor markers in colorectal cancer(CRC).Methods The morning urine and CRC tumor tissue were collected from 84 patients with CRC.The concentration of urine BPA was determined by liquid chromatography-mass spectrometer(LC-MS),urine BPA concentration was corrected with creatinine(Cr).Cer concentration of CRC tumor tissue was detected by Enzyme-linked immunosorbent assay(ELISA).The correlations of urine BPAcr,Cer content of CRC tumor tissue and tumor markers were analyzed.Results Cer content in CRC tumor tissue was positively correlated with BPAcr(r=0.784,P<0.001).Regression analysis showed that the regression coefficient of Cer content in CRC tumor tissue and BPAcr was 0.218(95%CI:0.18-0.26),which was statistically significant(P<0.001).There were significantly differences in CRC tumor tissue Cer and urine BPAcr between the CEA positive and negative groups,CA125 positive and negative groups,and CA19-9 positive and negative groups(all P<0.05),while there was no significant difference between AFP positive and negative groups in CRC tumor tissue Cer and urine BPAcr(P=0.247).Serum CEA,CA125 and CA19-9 were positively correlated with urine BPAcr(r values were 0.348,0.251,0.281,respectively,all P<0.05)and Cer content in CRC tumor tissue(r values were 0.265,0.309,0.263,respectively,all P<0.05).Conclusions BPA exposure may cause an increase of Cer in CRC tumor tissue and abnormalities in serum tumor markers,suggesting that BPA exposure may participate in the development and occurance of CRC by affecting the metabolism of Cer in CRC tumor tissue.
作者
吴明
张鑫东
袁少云
刘省存
沈彤
WU Ming;ZHANG Xin-dong;YUAN Shao-yun;LIU Sheng-cun;SHEN Tong(Department of Occupational Health and Environmental Health,School of Public Health,Anhui Medical University,Hefei 230032,China;Gastrointestinal Surgery,First Affiliated Hospital of Anhui Medical University,Hefei 230022,China)
出处
《中华疾病控制杂志》
CAS
CSCD
北大核心
2020年第1期26-30,共5页
Chinese Journal of Disease Control & Prevention
基金
国家自然科学基金(81773467)~~
