摘要
以超导量点为荧光探针,建立了一种超导量点-免疫荧光快速测定粮食中黄曲霉毒素B1的方法。采用多种方式评价了该方法与高效液相色谱法及酶联免疫吸附法检测稻谷及玉米样品的结果一致性,以验证该方法的准确性,同时对该方法的重复性和台间差异进行了考察。结果表明,超导量点-免疫荧光法对稻谷和玉米等粮食样品的检测结果与高效液相色谱法和酶联免疫法一致,表明该方法准确可靠,同时该方法两台仪器间的检测结果无显著性差异,且重复性好。
A method for rapid determination of aflatoxin Bi m grain based on QD-Infinity immunofluorescence was established with QD-Infinity as fluorescent probe.In order to confirm the accuracy of this method,multiple methods were used to evaluate the uniformity of the test results of AFBi in grain which was determined by the QD-Infinity immunofluorescence method,high performance liquid chromatography and enzyme association im-munoadsorption,respectively.Moreover the repeatability of the method and the difference between stations were investigated.The detection results of the superconducting point-immunofluorescence method for grain samples such as rice and corn were consistent with those of high performance liquid chromatography and enzyme association immunoadsorption,indicated that the method was accurate and reliable.There was no significant difference in the detection results when using two different instruments,which inclined a good repeatability of the method was good too.
作者
胡元斌
许艳霞
王达能
马志
倪小英
唐颜苹
余杨
HU Yuan-bin;XU Yan-xia;WANG Da-neng;MA-Zhi;NI Xiao-ying;TANG Yan-ping;YU Yang(Hunan Provincial Center for Monitoring of Grain Oil Products Quality,Changsha,Hunan 410201,China;Shenzhen Sitechno Biotechnology Co.,Ltd.,Shenzhen,Guangdong 518000,China)
出处
《食品与机械》
北大核心
2020年第1期84-87,109,共5页
Food and Machinery
基金
粮食公益性行业科研专项经费项目(编号：201513006-4)
