摘要
目的探讨非酒精性脂肪性肝病(NAFLD)小鼠肝组织脂质代谢相关基因水平变化。方法采用高脂饲料喂养20小鼠,构建小鼠NAFLD动物模型,观察小鼠肝组织病理学变化,检测血清总胆固醇(TCH)和甘油三酯(TG)含量,采用RT-PCR法检测小鼠肝组织胆固醇调节元件结合蛋白(SREBPs)、乙酰辅酶A羧化酶(ACC)和脂肪酸合成酶(fatty acid synthase,FAS)基因变化。结果10只NAFLD模型小鼠肝细胞排列不整齐,肝细胞内出现大小不等的脂滴;NAFLD模型小鼠血清TG和TCH水平分别为(0.63±0.13)mmol/L和(7.23±0.7)mmol/L,显著高于10只对照组[(0.28±0.06)mmol/L和(2.78±0.6)mmol/L,P<0.001];在实验24 w末,NAFLD组小鼠肝组织FAS和SREBP-1 mRNA水平分别为(3.9±1.1)和(1.8±0.7),对照组则分别为[(1.0±0.3)和(1.0±0.4),FAS:t=6.231,P<0.001;SREBP-1:t=2.431,P=0.035],差异显著,而NAFLD组小鼠肝组织ACC mRNA水平为(1.2±0.5),与对照组[(1.0±0.4),t=0.765,P=0.462]比,无显著差异。结论NAFLD模型小鼠肝组织与脂质代谢相关基因显著上调,为进一步研究NAFLD的发生发展机制奠定了基础。
Objective The aim of this experiment was to investigate the changes of lipid metabolism related genes in liver tissues of mice with nonalcoholic fatty liver diseases(NAFLD).Methods A NAFLD model was established in ten mice by feeding high-fat diet.The histopathological changes of the liver tissues were observed.Blood total cholesterol(TCH)and triglycerides(TG)levels were detected,and sterol regulatory element-binding proteins(SREBPs),acetyl coenzyme carboxylase(ACC)and fatty acid synthase(FAS)mRNA were detected by reverse transcription-polymerase chain reaction.Results The steatosis of liver cells was found in mice in NAFLD model group;blood TG and TCH levels in NAFLD model group were(0.63±0.13)mmol/L and(7.23±0.7)mmol/L,significantly higher than[(0.28±0.06)mmol/L and(2.78±0.6)mmol/L,P<0.001]in ten mice in the control;at the end of 24 w experiment,hepatic FAS and SREBP-1 mRNA in NAFLD mice were(3.9±1.1)and(1.8±0.7),significantly higher than[(1.0±0.3)and(1.0±0.4),FAS:t=6.231,P<0.001;SREBP-1:t=2.431,P=0.035],while the ACC mRNA was(1.2±0.5),not significantly different as compared to[(1.0±0.4),t=0.765,P=0.462]in the control.Conclusion The lipid metabolism related genes in liver tissue of mice with NAFLD are significantly up-regulated,which might play a pivotal role in the pathogenesis of NAFLD.
作者
张猛
陈晹
刘娇
李叶晟
黄杨卿
Zhang Meng;Chen Yi;Liu Jiao(Department of Hepatobiliary Surgery,Public Health Clinical Center,Fudan University,Shanghai 201508,China)
出处
《实用肝脏病杂志》
CAS
2020年第2期163-166,共4页
Journal of Practical Hepatology
基金
上海市卫生和计划生育委员会科研项目(编号:201840128)
关键词
非酒精性脂肪性肝病
脂质代谢
脂肪酸合成酶
胆固醇调节元件结合蛋白
小鼠
Nonalcoholic fatty liver
Lipid metabolism
Sterol regulatory element-binding proteins
Acetyl coenzyme carboxylase
Fatty acid synthase
Mice
