摘要
目的通过掺入饲料的方式连续给予SD大鼠转cry2A^*基因大米T2A-152周,为其食用安全性提供基础毒理学数据。方法144只无特定病原体(SPF级)SD大鼠按体质量随机分为三个组,每组雌雄各半。根据大米营养成分测定结果,将T2A-1大米及其亲本明恢63大米均按最大比例掺入饲料中,成长期和维持期分别为60.75%、66.75%,同时设置AIN-93配方饲料组作为营养对照。连续喂饲52周;试验期间观察并记录一般状况、体质量、摄食量等,并开展血液、血生化、尿液常规检测;试验结束时处死动物进行病理学检查等。结果转基因和亲本大米的主要营养成分含量相近。52周试验周期中,各组大鼠一般状况均良好,死亡率和肿瘤发生率组间差异无统计学意义(P>0.05)。与非转基因组比较,转基因组大鼠体质量、进食量、食物利用率差异无统计学意义(P>0.05);血液、血生化、尿液检测未发现有毒理学意义的改变;各组组织病理学检查均未发现有意义的病理学改变。结论在本试验条件下,转cry2A^*基因大米连续喂饲SD大鼠52周,未发现其对实验动物产生不良的影响。
Objective In the present work,the chronic toxic effects of the transgenic rice T2 A-1 carrying cry2 A^*gene was evaluated on Sprague-Dwaley rats through a 52-week feeding study.Methods One hundred and forty-four rats were randomly divided into 3 groups according to body weight.Transgenic rice T2 A-1 and parent rice were formulated into diets at high levels of 60.75%during the growth period and 66.75%during the maintenance period,respectively.AIN-93 diet was used as a nutritional control.Animals were fed with the corresponding feed for 52 weeks.Cage-side observation,animal body weight,food intake,food utilization,hematology,serum biochemistry and urine analysis were carried out routinely.At the end of the administration,all survived animals were anatomized and histopathological examination were conducted.Results The main nutrient contents of the transgenic rice were similar to the parent rice.Throughout the experimental period,the trends in body weight,food intake and food utilization rate were similar in all groups.Although statistical significance was observed in some indexes of hematology,serum biochemistry and urine analysis of the transgenic rice groups as compared with the parent rice group,they were not considered as treatment-related.Histopathological examination showed some spontaneous lesions in all groups with no significant difference among them.Conclusion The result of the current 52-weeks chronic toxicity study of transgenic rice T2 A-1 exerted no unintended adverse effects on rats.
作者
唐晓荞
王杨凤
裴兰洁
杨文祥
樊军
卓勤
杨晓光
刘家发
樊柏林
TANG Xiaoqiao;WANG Yangfeng;PEI Lanjie;YANG Wenxiang;FAN Jun;ZHUO Qin;YANG Xiaoguang;LIU Jiafa;FAN Bolin(Hubei Provincial Key Laboratory for Applied Toxicology Hubei Provinctial Center for Disease Control and Prevention,Hubei Wuhan 430079,China;Ruling District Center for Disease Control and Prevention,Chongqing 408000,China;National Institute for Nutrition and Food Safety,Chinese Center for Disease Control and Prevention,Beijing 100050,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2019年第6期510-516,共7页
Chinese Journal of Food Hygiene
基金
农业部“转基因生物新品种培育”科技重大专项资助(2016ZX08011005)
湖北省领军人才培养工程专项经费
关键词
转基因水稻
慢性毒性
安全性评价
Transgenic rice
chronic toxicity
safety evaluation
