摘要
目的 体外构建丝素蛋白(silk fibroin,SF)、I型胶原(type I collagen,Col-I)和羟基磷灰石(hydroxyapatite,HA)共混体系制备二维复合膜和三维仿生支架,研究其理化性质和生物相容性,探讨其在组织工程支架材料中应用的可行性。方法 通过在细胞培养小室底部共混SF/Col-I/HA以及低温3D打印结合真空冷冻干燥法制备二维复合膜及三维支架。通过机械性能测试、电子显微镜和Micro-CT检测材料的理化性质,检测细胞的增殖评估其生物相容性。结果 通过共混和低温3D打印获得稳定的二维复合膜及三维多孔结构支架;力学性能具有较好的一致性,孔径、吸水率、孔隙率和弹性模量均符合构建组织工程骨的要求;支架为网格状的白色立方体,内部孔隙连通性较好;HA均匀分布在复合膜中,细胞黏附在复合膜上,呈扁平状;细胞分布在支架孔壁周围,呈梭形状,生长及增殖良好。结论 利用SF/Col-I/HA共混体系成功制备复合膜及三维支架,具有较好的孔连通性与孔结构,有利于细胞和组织的生长以及营养输送,其理化性能以及生物相容性符合骨组织工程生物材料的要求。
Objective To construct a two-dimensional(2D)composite membrane and a three-dimensional(3D)biomimetic scaffold by silk fibroin(SF),typeⅠcollagen(Col-I)and hydroxyapatite(HA)blends in vitro,so as to study its physicochemical properties,as well as biocompatibility and explore the feasibility of its application in tissue engineering scaffold materials.Methods 2D composite membranes and 3D scaffolds were prepared by blending SF/Col-I/HA at the bottom of cell culture chamber and low temperature 3D printing combined with vacuum freeze drying.The biocompatibility was evaluated by mechanical property testing,scanning electron microscope and Micro-CT to examine the physicochemical properties of the material,and cell proliferation was detected to evaluate its biocompatibility.Results Stable 2D composite membrane and 3D porous structural scaffolds were obtained by blending and low temperature 3D printing.The mechanical properties were consistent.The pore size,water absorption,porosity and elastic modulus were all in accordance with the requirements of constructing tissue engineering bone.The scaffold was a grid-like white cube with good internal pore connectivity;HA was evenly distributed in the composite membrane,and the cells were attached to the composite membrane in a flat shape;the cells were distributed around pore walls of the scaffold.The shape of the shuttle was fusiform,and the growth and proliferation were good.Conclusions The composite membrane and 3D scaffold prepared by SF/Col-I/HA blending system had better pore connectivity and pore structure,which was beneficial to cell and tissue growth and nutrient transport.Its physicochemical properties and biocompatibility could meet the requirements of bone tissue engineering biomaterials.
作者
程威
张扬
宋秀钢
林祥龙
刘洋
李瑞欣
徐云强
张西正
CHENG Wei;ZHANG Yang;SONG Xiugang;LIN Xianglong;LIU Yang;LI Ruixin;XU Yunqiang;ZHANG Xizheng(Department of Orthopaedics,General Hospital of Tianjin Medical University,Tianjin 300052,China;Department of Clinical Medicine,Graduate School,Tianjin Medical University,Tianjin 300070,China;Institute of Medical Service and Technology,Academy of Military Sciences,Tianjin 300161,China;Tianjin Key Laboratory of Advanced Electromechanical System Design and Intelligent Control,Tianjin University of Technology,Tianjin 300041,China;Tianjin Stomatological Hospital Central Laboratory,Tianjin 300384,China)
出处
《医用生物力学》
EI
CAS
CSCD
北大核心
2019年第6期623-630,共8页
Journal of Medical Biomechanics
基金
国家自然科学基金项目(31470935,11432016)
关键词
仿生骨材料
复合膜
三维支架
多维结构
生物相容性
bionic bone materials
composite membrane
three-dimensional(3D)scaffold
multidimensional structure
biocompatibility
