摘要
Objective:To observe the lipid-lowering effect of different transdermal absorption enhancers applied to the herbal cake-partitioned moxibustion in hyperlipidemia model rabbits,and to explore the possible mechanism.Methods:Forty New-Zea I a nd rabbits were ran domly divided into 5 groups using the ran dom nu mber table method,with 8 rats in each group.Rabbits in the blank group were fed routinely with normal diet;rabbits in the other groups were fed with high-fat diet for 12 weeks to establish the hyperlipidemia model.Rabbits in the blank and the model groups were not treated.After the model was prepared,rabbits in the non-transdermal absorption enhancer group received herbal cake-partitioned moxibustion without transdermal absorption enhancer;rabbits in the laurocapram group and the borneol group received herbal cake-partitioned moxibustion with laurocapram or borneol respectively.After 4 weeks of treatment,serum was collected for enzyme-linked immunosorbent assay(ELISA),and the liver tissues were isolated for imm uno histochemistry,qua ntitative polymerase chain reactio n(qPCR)and Western-blotting(WB)detecti on.Results:Serum ELISA results showed that leptin was significantly decreased in the model group compared with the blank group(P<0.05);compared with the model group,lepti n was significa ntly in creased in the non-tran sdermal absorpti on enhanee。the laurocapram and the borneol groups(all P<0.05);compared with the non-transdermal absorption enhancer group,leptin was significantly increased in the laurocapram group and the borneol group(both P<0.05);there was no significant differenee in leptin between the laurocapram and the borneol groups(P>0.05).The qPCR results of rabbit liver tissues showed that the mRNA expressions of leptin,Janus kinase 2(JAK2)and signal transducer and activator of transcription 3(STOT3)in the model group were significantly lower than those in the blank group(all P<0.05);compared with the model group,the mRNA expressions of leptin,leptin receptor(LR),JAK2 and S1AT3 in the non-transde
目的:观察不同促透剂运用于隔药饼灸对高脂模型兔降脂效果的影响,探讨其可能机制。方法:将40只新西兰兔按随机数字表法随机分成5组,每组8只。空白组正常喂养普通饲料;其余组高脂饲料喂养12周,复制高脂模型。空白组和模型组不治疗。无促透剂组成模后采用不加促透剂药饼施灸;氮酮组和冰片组分别将氮酮和冰片用于药饼中,进行隔药饼灸。治疗4周后,分离血清进行酶联免疫吸附测定(ELISA),分离肝脏组织进行免疫组化、定量聚合酶链式反应(qPCR)和蛋白免疫印迹(WB)检测。结果:血清ELISA检测结果显示,与空白组比较,模型组Leptin显著下降(PV0.05);与模型组比较,无促透剂组、氮酮组和冰片组Leptin显著升高(均PV0.05);与无促透剂组比较,氮酮组和冰片组Leptin均显著升高(均PV0.05);氮酮组和冰片组Leptin差异无统计学意义(P>0.05)。兔肝脏组织qPCR结果显示,与空白组比较,模型组Leptin JAK2和STAT3 mRNA表达显著降低(均PV0.05);与模型组比较,无促透剂组、氮酮组和冰片组Leptin、Leptin受体、JAK2和STAT3 mRNA表达显著升高(均PV0.05);与无促透剂组相比,氮酮组和冰片组的Leptin.Leptin受体、JAK2和STAT3 mRNA表达显著升高(均PV0.05);与氮酮组比较,冰片组的Leptin、Leptin受体、JAK2和STAT3 mRNA表达显著升高(均PV0.05)。免疫组化和WB检测趋势与qPCR结果基本一致。磷酸化JAK2(Phospho-JAK2)和磷酸化STAT3(phospho-STAT3)免疫组化和WB检测趋势与JAK2和STAT3基本一致。结论:高脂模型兔的Leptin/JAK"STAT3通路分子表达下降,隔药饼灸后Leptin/JAK?/STAT3通路因子表达显著上升,氮酮和冰片作为促透剂运用于隔药饼灸比单纯隔药饼灸对Leptin/JAK刀STAT3调脂通路相关因子的上调作用更明显。
基金
湖南省高校创新平台开放基金项 @, No. 16K068
2017年湖南省研究生科研创新项目,No.CX2017B431。
