摘要
目的研究去铁胺(DFO)对高氧机械通气大鼠肺表面活性蛋白D(SP-D)和抗氧化酶的影响。方法雄性SD大鼠24只,体重180~220 g。随机分为三组:空气组(M组)、高氧组(H组)和DFO组(D组),每组8只。麻醉状态下,M组行空气(FiO2=21%)机械通气,H组和D组行高氧(FiO2=90%)机械通气。M组和H组尾静脉输注生理盐水1 ml/h,D组尾静脉输注等容量DFO(200mg/kg,1 ml/h)。机械通气4 h后取左肺制备支气管肺泡灌洗液(BALF),取右肺组织制备肺组织匀浆、计算湿干重比(W/D)并制备病理切片、行病理损伤评分。采用ELISA法测定BALF和肺组织匀浆中SP-D含量、谷胱甘肽还原酶(GR)和黄嘌呤氧化酶(XOD)活性。结果H组和D组BALF和肺组织匀浆中SP-D含量明显低于M组(P<0.05),GR活性明显弱于M组(P<0.05),XOD活性明显强于M组(P<0.05),W/D和病理损伤评分明显高于M组(P<0.05)。D组SP-D含量明显高于H组(P<0.05),GR活性明显强于H组(P<0.05),XOD活性明显弱于H组(P<0.05),W/D和病理损伤评分明显低于H组(P<0.05)。结论高氧机械通气4 h可造成明显的肺损伤和肺组织SP-D含量降低,去铁胺可增加SP-D含量、减轻肺损伤,其机制与增强肺组织GR活性和降低XOD活性有关。
Objective To investigate the effect of deferoxamine(DFO)on pulmonary surfactant protein D(SP-D)and antioxidant enzymes in rats exposed to hyperoxia ventilation.Methods Twenty-four male sprague dawley rats,weighing 180-220 g,were randomly divided into 3 groups(n=8):air group(group M),hyperoxia group(group H)and DFO group(group D).The rats in group M were ventilated with air(FiO2=21%)and the rats in groups H and D were ventilated with oxygen(FiO2=90%)for 4 h.Meanwhile,normal saline 1 ml/h was infused via tail vein in groups M and H.The rats in group D were infused intravenously with the same volume of DFO(200 mg/kg,1 ml/h).Bronchoalveolar lavage fluid(BALF)was prepared in left lung after 4 h of ventilation.The right lung was taken to make tissue homogenate,the wet/dry weight(W/D)ratio was measured,the pathological changes was observed and the injury level was evaluated.The content of pulmonary surfactant protein D(SP-D),the activity of glutathione reductase(GR)and xanthine oxidase(XOD)were detected in BALF and lung homogenate by ELISA.Results The content of SP-D and the activity of GR in groups H and D were much lower than those in group M(P<0.05).The activity of XOD,lung W/D and pathological evaluation in groups H and D were much higher than those in group M(P<0.05).The content of SP-D and the activity of GR in group D were higher than those in group H(P<0.05).Meanwhile,the activity of XOD,lung W/D and pathological evaluation in group D were significantly lower than those in group H(P<0.05).Conclusion Four hours of hyperoxia ventilation causes significant lung injury and SP-D content reduction in lung tissue.Deferoxamine infusion has an effect of increasing SP-D content and alleviating lung injury,and the mechanism is related to the increased GR and decreased XOD activity.
作者
沙小兰
李玉兰
王晓霞
王晶晶
陈苏衡
SHA Xiaolan;LI Yulan;WANG Xiaoxia;WANG Jingjing;CHEN Suheng(Department of Anesthesiology,the First Medical College of Lanzhou University,Lanzhou 730000,China)
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2019年第10期1002-1005,共4页
Journal of Clinical Anesthesiology
关键词
去铁胺
肺表面活性蛋白D
高氧
机械通气
肺损伤
Deferoxamine
Pulmonary surfactant protein D
Hyperoxia
Ventilation
Lung injury
