摘要
通过热诱导法制备EGCG-β-LG纳米粒,并通过单因素实验考察β-LG浓度、EGCG浓度、pH、热激温度对EGCG-β-LG纳米粒包封率的影响。按照优化后的处方配比制备EGCG-β-LG纳米粒,以保护EGCG生物活性,提高EGCG体外稳定性,并测定其包封率、粒径、Zeta电位、体外稳定性及体外释放率。结果表明pH为6.5、热激温度75℃、β-LG浓度3mg/mL、EGCG浓度0.8mg/mL是制备EGCG-β-LG纳米粒的最佳条件。EGCG-β-LG纳米粒包封率为82.61%、粒径为94.46nm、Zeta电位为-27.9mV。EGCG-β-LG纳米粒的EGCG保留率高于EGCG水溶液,其在pH5.5和pH7.4的体外释放率皆低于EGCG水溶液。制备得到的EGCG-β-LG纳米粒体外稳定性较EGCG水溶液有所提高,并有一定的缓释性能。
Objective:EGCG-β-LG nanoparticles were constructed to protect the biological activity and stability of EGCG in vitro.Methods:Preparation of EGCG-β-LG nanoparticles by thermal induction method.The effects ofβ-LG concentration,EGCG concentration,pH and heat shock temperature on the encapsulation efficiency of EGCG nanoparticles were investigated by single factor experiments.EGCG-β-LG nanoparticles were prepared according to the optimized formula.The encapsulation efficiency,particle size,zeta potential,stability and release rate were measured.Results:The conditions for the preparation of EGCG-β-LG nanoparticles are as follows:pH 6.5,heat shock temperature 75℃,β-LG concentration was 3mg/mL,EGCG concentration was 0.8mg/mL.The encapsulation efficiency of EGCG-β-LG nanoparticles was 82.61%,the particle size was 94.46 nm,and the zeta potential was-27.9 mV.The retention of EGCG in EGCG-β-LG nanoparticles was higher than that in EGCG aqueous solution,and the release rates of EGCG in pH5.5 and pH7.4 were lower than that in EGCG aqueous solution.Conclusion:EGCG-β-LG nanoparticles were prepared.The stability of the nanoparticles in vitro was better than that of EGCG aqueous solution,and the nanoparticles had a certain sustained-release performance.
作者
孙陶利
周芫宇
黎绫
Sun Tao-li;Zhou Yuan-yu;Li ling(School of Pharmacy,Changsha Medical College,Hunan key laboratory cultivation base of the research and development of novel pharmaceutical preparations,Hunan Changsha 410219)
出处
《生物化工》
2020年第1期35-37,54,共4页
Biological Chemical Engineering
基金
湖南省教育厅一般项目（17C0171）
湖南省教育厅一般项目（17C0168）
湖南省自然科学基金青年项目（2018JJ3571）
