摘要
基因编辑技术是研究基因功能和作物改良品种的有力工具。该技术依靠工程内切酶在目标位点产生双链断裂(double strand DNA breaks, DSB)。随后双链断裂通过非同源末端连接(non-homologous end-joining,NEHJ)和同源重组(homology-directed repair, HDR)两种途径修复,分别导致突变和序列替换。目前应用最广泛的基因编辑技术是CRISPR-Cas9系统,它主要由Cas9蛋白和单链RNA组成,在RNA的引导下对目标基因进行编辑。本综述简要介绍基因编辑技术的发展,侧重于CRISPR-Cas9系统的发展以及其在基因功能和作物育种中的最新研究进展。
Gene editing is a powerful tool for studying gene function and crop improvement varieties. This technique relies on engineering endonuclease to produce double strand breaks(DSB) at target sites. Subsequently,double strand breaks were repaired via non-homologous end-joining(NEHJ) or homology-directed repair(HDR),resulting in mutation and sequence replacement, respectively. At present, the most widely used gene editing technique is CRISPR-Cas9 system, which is composed of Cas9 protein and single-stranded RNA. Under the guidance of RNA, the target gene is edited. This paper briefly introduces the development of gene editing technology, focusing on the development of CRISPR-Cas9 system and its latest research progress in gene function and crop breeding.
作者
赵山山
邸一桓
郝光飞
Zhao Shanshan;Di Yihuan;Hao Guangfei(College of Life Sciences and Food Engineering,Hebei University of Engineering,Handan,056000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第21期7087-7093,共7页
Molecular Plant Breeding
基金
河北省自然科学基金资助项目(C2016402014)
河北省高等学校科学技术研究项目(QN2017033)
邯郸市科学技术研究与发展计划项目(1622201049)共同资助
