摘要
建立一种基于竞争形式的灵敏、稳定的表面等离子体共振免疫传感器,并用于检测饲料和水产养殖水样中的喹乙醇(olaquindox,OLA)。根据本研究的方法,OLA的灵敏度和检出限分别为4.7μg/L和0.5μg/L。用2.5 mmol/L NaOH溶液再生效果最好,芯片可重复利用60次,检测时间为10 min。OLA与结构类似物的交叉反应结果显示了高灵敏度和良好特异性。在鱼、虾饲料和水产养殖水样的添加回收实验中均获得良好的回收率(84.7%~99.3%)和变异系数(1.2%~10.1%)。对饲料和养殖水实际样品进行测定,结果检测到2个阳性样品,鱼养殖水与鸡饲料中OLA质量浓度分别为5.0、6.1μg/L。该方法可以高效地检测大批量饲料和水样的OLA,具有检测时间短、检出限低、准确灵敏、操作简便等优点。
A sensitive and stable competitive surface plasmon resonance(SPR)immunosensor was established and used to detect olaquindox(OLA)in feed and aquaculture water samples.Its sensitivity and limit of detection(LOD)were 4.7 and 0.5μg/L,respectively.The best regeneration efficiency was obtained with 2.5 mmol/L NaOH solution,the chip could be reused 60 times and the detection time was only 10 min.The results of cross-reaction between OLA and structural analogs showed a high sensitivity and specificity.Additionally,good recoveries(84.7%–99.3%)and coefficients of variation(1.2%–10.1%)were obtained for spiked fish,shrimp feed and aquaculture water samples.When the method was applied to test actual samples of feed and culture water,two positive samples were detected.OLA contents in fish culture water and chicken feed were 5.0 and 6.1μg/L,respectively.The method can efficiently detect OLA in large-volume feed and water samples,and has the advantages of short detection time,low detection limit,high accuracy and sensitivity,and convenient operation.
作者
谢春花
柳双
王敏思
王霞琳
王珅
张志军
宋洋
XIE Chunhua;LIU Shuang;WANG Minsi;WANG Xialin;WANG Shen;ZHANG Zhijun;SONG Yang(Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;National Engineering and Technology Research Center for Preservation of Agricultural Products(Tianjin),Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Tianjin 300384,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第4期293-299,共7页
Food Science
基金
天津市自然科学基金项目(18JCQNJC84400)
国家自然科学基金青年科学基金项目(31301487)
天津市科技计划项目(13JCQNJC14800
14ZCDGNC00098)
