摘要
目的探讨P-糖蛋白表达与非肌层浸润性膀胱癌(NMIBC)患者术后复发的关系。方法选择接受经尿道膀胱肿瘤电切术(TURBT)治疗的47例NMIBC患者,所有患者均于术后单一应用吡柔比星规律腔内灌注1年或至治疗失败。术后随访1年,10例患者复发,37例患者未复发。取初治且随访1年未复发患者的手术切除标本为A组(n=37),初治且随访1年复发患者的第1次手术切除标本为B组(n=10),复发患者的再次及多次手术切除标本为C组(n=12)。采用免疫组织化学染色法检测各组中P-糖蛋白的表达情况,比较不同组别NMIBC组织中P-糖蛋白的表达情况,分析P-糖蛋白表达与NMIBC组织学分级的相关性。结果C组中P-糖蛋白的染色评分高于(A+B)组,差异有统计学意义(P﹤0.05);A组和B组中P-糖蛋白的染色评分比较,差异无统计学意义(P﹥0.05)。P-糖蛋白表达与NMIBC的组织学分级无相关性(P﹥0.05)。结论灌注化疗后NMIBC组织中P-糖蛋白表达上调,但不能将P-糖蛋白作为TURBT术后NMIBC是否复发的预测指标,P-糖蛋白表达与NMIBC的组织学分级无相关性。
Objective To investigate the relationship between the expression of P-glycoprotein and the postoperative recurrence of non-muscle invasive bladder cancer(NMIBC).Method The study included 47 cases of NMIBC after transurethral resection of bladder tumor(TURBT),who received regular intravesical instillation of pirarubicin monotherapy for 1 year or till treatment failure.All patients were followed up for 1 year after surgery,10 cases had recurrence while another 37 ones had not.Specimens from patients receiving first-time treatment and followed up for 1 year without recurrence were collected as group A(n=37),meanwhile,specimens from those who received first-time treatment and followed up for 1 year with recurrence were collected during their first surgical resection as group B(n=10),finally,specimens collected during the second or subsequent multiple surgical resections from patients with recurrent disease were included as group C(n=12).Immunohistochemical staining was used to detect the expression of P-glycoprotein in each group of specimens,to determine the differences of P-glycoprotein expression,and the correlation between P-glycoprotein expression and histological grade of NMIBC was also explored.Result The staining score of P-glycoprotein in group C was significantly higher than group(A+B),with statistically significant difference observed(P<0.05);while the scores of group A and B were similar(P>0.05).There was no significant correlation between P-glycoprotein expression and the histological grade of NIMBC(P>0.05).Conclusion The expression of P-glycoprotein in NMIBC tissues is upregulated after intravesical instillation of pirarubicin.However,P-glycoprotein cannot be used as a predictor for recurrence of NMIBC after TURBT,and there is no evidence for a significant correlation between P-glycoprotein expression and the histological grade of NMIBC.
作者
陈美元
张颖
冯建伟
郎金田
崔刚
张志国
CHEN Meiyuan;ZHANG Ying;FENG Jianwei;LANG Jintian;CUI Gang;ZHANG Zhiguo(Department of Urologic Surgery,Renmin Hospital of Daxing District,Beijing 102600,China;Department of Medical Oncology,Renmin Hospital of Daxing District,Beijing 102600,China)
出处
《癌症进展》
2020年第1期62-64,共3页
Oncology Progress
基金
北京市大兴区科技发展计划项目课题(KT2015000153)
关键词
非肌层浸润性膀胱癌
P-糖蛋白
免疫组织化学染色
多药耐药
non-muscle invasive bladder cancer
P-glycoprotein
immunohistochemical staining
multidrug resistance
