摘要
植物化学调控技术是提高作物产量的一项重要的技术措施,研究植物生长调节剂2-N,N-二乙氨基乙基己酸酯(DTA-6)对甜叶菊形态建成、酶活性、莱鲍迪甙A与甜菊醇糖苷比值(ReA/ST)的影响,目的是为调节剂DTA-6提高甜叶菊产量、品质的调控效应及机理提供理论依据。试验于2017年在黑龙江省海林市海林农场试验基地进行,用大田试验方法,在8月15日(移栽后75天后)喷施不同浓度DTA-60 mg·L^-1(CK)、12.5 mg·L^-1(D12.5)、60 mg·L^-1(D60)、300 mg·L^-1(D300),在8月20日、9月7日、9月22日(分别为移栽后80、98、113 d)进行取样,测定甜叶菊的形态、酶活性及ReA/ST,收获时测定产量。结果表明,在8月20日:D12.5处理的株高、茎粗、分枝数、POD酶活性和ReA/ST与CK相比均极显著增加,PPO酶活性极与CK相比显著降低;D60处理的POD酶活性与CK相比均极显著增加,PPO酶活性和ReA/ST与CK相比均极显著降低;D300处理的分枝数、POD酶活性、ReA/ST与CK相比均极显著增加。在9月7日:D12.5、D60、D300处理的PPO酶活性和ReA/ST与CK相比均极显著降低,POD酶活性与CK相比均极显著增加。在9月22日:D12.5处理的茎粗和POD酶活性与CK相比极显著增加,分枝数与CK相比显著增加,PPO酶活性极显著降低;D60处理的株高、分枝数、单株茎重和ReA/ST与CK相比均极显著增加,PPO酶活性极显著降低、POD酶活性显著降低;D300处理的PPO酶活性和与POD酶活性CK相比极显著降低,ReA/ST极显著增加。收获时,D12.5处理产量增加了3.8%;D60处理产量增加了11.2%,并且差异达到了显著水平;D300处理产量增加了8.0%。综上所述,试验所设计浓度范围中60 mg·L^-1 DTA-6浓度对甜叶菊的调控效果最为显著。
Plant chemical regulation technology was an important technical method to increase crop yield.The effect of plant growth regulator 2-N,N-diethyl amino ethyl caproate(DTA-6)on the morphogenesis,enzyme activity,the ratio of rebaudioside A to stevioside(ReA/ST)of stevia was studied.The purpose was to provide theoretical basis for the regulation effect of DTA-6 on the yield and quality of stevia.The experiment was carried out in 2017 at experimental research station in Hailin,Heilongjiang province.Different concentrations of DTA-60 mg·L^-1(CK),12.5 mg·L^-1(D12.5),60 mg·L^-1(D60),300 mg·L^-1(D300)were sprayed on the leaves of Stevia on August 15(75 days after transplantation)in the field.Samples were taken on August 20,September 7 and September 22(80,98 d and 113 d after transplantation respectively)to determine the morphology,enzyme activity and ReA/ST of stevia,and the yield was determined at harvest.The results showed,on August 20:in comparison with CK,the plant height,stem diameter,branch number,POD enzyme activity and ReA/ST were significantly increased(P<0.01)and PPO enzyme activity was remarkably decreased in the treatment of D12.5;POD enzyme activity under D60 treatment was clearly increased(P<0.01).However,PPO enzyme activity and ReA/ST were obviously decreased(P<0.01).The number of branches,POD enzyme activity and ReA/ST under D300 treatment were significantly increased(P<0.01)compared with CK.On September 7:PPO enzyme activity and ReA/ST activity in the treatment of D12.5,D60 and D300 were all remarkably decreased(P<0.01),and POD enzyme activity was significantly increased(P<0.01)compared with CK.On September 22:comparing with CK,the POD enzyme activity and stem diameter of D12.5 treatment were clearly increased(P<0.01)and the number of branches was distinctly increased,while,PPO enzyme activity was significantly decreased(P<0.01).In contrast to CK,the plant height,branch number,single plant stem weight and ReA/ST under D60 treatment were all significantly improved(P<0.01),meanwhile,PPO enzyme activity
作者
冯胜杰
王新欣
贾鹏宇
王诗雅
卢洁春
左官强
刘雅
黄文婷
冯乃杰
郑殿峰
Feng Shengjie;Wang Xinxin;Jia Pengyu;Wang Shiya;Lu Jiechun;Zuo Guanqiang;Liu Ya;Huang Wenting;Feng Naijie;Zheng Dianfeng(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Agronomy,Guangdong Ocean University;National Coarse Cereals Engineering Research Center)
出处
《黑龙江八一农垦大学学报》
2020年第1期10-16,45,共8页
journal of heilongjiang bayi agricultural university
基金
黑龙江农垦总局(复合型植物生长调节剂的研究与应用:HNK12A-06-03)资助