摘要
目的:观察用6-羟基多巴胺(6-OHDA)诱导的帕金森病(PD)模型大鼠中腹膜后白色脂肪组织(rWAT)内脂肪酸合成酶(FAS)、激素敏感性脂肪酶(HSL)和磷酸化激素敏感性脂肪酶(p-HSL)的变化。方法:20只SD大鼠随机分为PD模型组(双侧黑质内注射6-OHDA)和对照组(注射等体积生理盐水)。每日测量进食量,手术后6周进行转棒实验,并测量体重和rWAT重量。免疫组织化学染色观察黑质中的酪氨酸羟化酶(TH)的表达变化。HE染色观察rWAT中脂肪细胞的形态改变,并用ImageJ软件测量脂肪细胞的直径。Western blot检测黑质中TH及rWAT中FAS、HSL和p-HSL的蛋白水平。结果:与对照组比较,PD模型大鼠黑质内阳性神经元显著减少,运动能力明显下降;每日进食量和体重均无明显变化,但rWAT重量/体重的比值明显降低(P<0.05)。rWAT中脂肪细胞直径明显减少。rWAT中FAS表达明显降低,p-HSL的水平明显升高,HSL变化的差异无统计学显著性。结论:黑质多巴胺参与大鼠rWAT的合成与分解调控。黑质破坏引起的rWAT代谢改变可能与PD病人体重下降具有一定的相关性。
AIM:To observe the alterations of fatty acid synthase(FAS),hormone-sensitive lipase(HSL)and phosphorylated HSL(p-HSL)in retroperitoneal white adipose tissue(rWAT)in a 6-hydroxydopamine(6-OHDA)-induced Parkinson disease(PD)rat model.METHODS:SD rats(n=20)were randomly divided into PD model group and control group.6-OHDA was injected into bilateral substantia nigra(SN)of the rats in PD model group,and the same volume of saline was injected into the same position of the rats in control group.Food intake was measured daily.Six weeks after operation,rotarod test was performed,and the body weight and rWAT weight of the rats were recorded.Immunohistochemistry was used to observed the change of tyrosine hydroxylase(TH)in SN.HE staining was used to observe the morphological change of adipocytes in rWAT,and the diameter of adipocytes was measured by ImageJ software.The protein levels of TH in SN,and FAS,p-HSL and HSL in rWAT were determined by Western blot.RESULTS:Compared with control group,TH-positive neurons in SN were significantly reduced,and the motor ability of PD model rats was significantly decreased.No obvious change of daily food intake and body weight was observed,but the ratio of rWAT weight/body weight and the diameter of adipocytes in rWAT of PD model rats were significantly decreased.The protein level of FAS was decreased significantly.The protein level of p-HSL was increased significantly,while the protein level of HSL did not change.CONCLUSION:Dopamine in SN is involved in the regulation of rWAT synthesis and lipolysis in rats.The metabolic changes of rWAT caused by the destroy of SN may be related to the weight loss of PD patients.
作者
连辉
周立
张毅敏
宋海岩
王志勇
LIAN Hui;ZHOU Li;ZHANG Yi-min;SONG Hai-yan;WANG Zhi-yong(Department of Human Anatomy,Xinxiang Medical University,Xinxiang 453003,China;Laboratory of Human Anatomy,Xinxiang Medical University,Xinxiang 453003,China;Xinxiang Key Laboratory of Molecular Neurology,Xinxiang Medical University,Xinxiang 453003,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2020年第2期289-293,共5页
Chinese Journal of Pathophysiology
基金
河南省高校青年骨干教师资助计划(No.2017GGJS107)
河南省医学组织再生重点实验室开放课题(No.KFKT16003)
新乡医学院博士科研启动基金资助项目(No.XXBSKYZZ201501)
