摘要
目的探讨女性泌尿道宿主、环境、微生物菌群间的关系及宿主反应性变化,证明泌尿道存在特征性菌群;探讨菌群变化与尿路感染的关系,对尿路感染诊疗产生观念性改变。方法临床资料收集于2018年2月至2018年3月期间,应用16SrRNA基因测序对健康绝经后(A组,5例)、健康育龄期(B组,5例)及尿路感染的绝经后女性(C组,14例)尿液进行菌群测定,分析泌尿道菌群结构和组成的多样性及差异性。结果女性尿液中包含种类繁多的微生物菌群,构成多样化,个体差异明显,一般以一种或两种菌种占优势,健康育龄期、绝经后女性组优势菌种主要为乳酸杆菌属、加德纳菌属及鞘氨醇单胞菌属,而鞘氨醇单胞菌属在尿路感染绝经后女性组丰度则明显下降;同时,尿路感染组优势菌群主要为肠杆菌科及乳酸杆菌属,且肠杆菌科丰度明显高于另外两组;而乳酸杆菌属丰度水平在健康育龄期组则明显高于绝经后两组。结论女性泌尿道菌群以乳酸杆菌属为优势菌,绝经后尿路感染女性泌尿道菌群表现为乳酸杆菌属、鞘氨醇单胞菌属显著减少及肠杆菌科丰度增加,提示这可能是引起绝经后妇女尿路感染发病率升高的重要原因,为诊断尿路感染及非抗菌素手段治疗尿路感染提供思路。
Objective To explore the relationship between female urinary tract host,environment,microbial flora and changes in the host's suitability,and to prove that the urinary tract has its characteristic flora.To investigate the relationship between bacterial flora changes and urinary tract infections,and to make conceptual changes in the diagnosis and treatment of urinary tract infections.Methods From February 2018 to March 2018,16SrRNA gene sequencing was used to detect the urine samples of women of healthy postmenopausal group(n=5),healthy childbearing age group(n=5),and postmenopausal with urinary tract infection group(n=14),and the diversity and diversity of urogenital microbiome were analyzed.Results The female urine contains a wide variety of microbiome,and the microbiome constitutes a diversity of individual differences,generally one or two strains predominates.The dominant bacteria in the healthy childbearing age and postmenopausal group are mainly lactobacillus,gardnerella,and sphingomonas,while sphingomonas in the postmenopausal urinary tract infection group was abruptly decreased in abundance.In postmenopausal with urinary tract infections,the predominant microbiome was mainly enterobacteriaceae and lactobacillus,and the abundance of enterobacteriaceae was significantly higher than other two groups,while the abundance of lactobacilli was significantly higher in the healthy childbearing age group than in the postmenopausal period two groups.Conclusions The dominant urobacteria of the female urogenital are lactobacillus.The urinary microbiome of women with postmenopausal urinary tract infection shows a significant reduction in lactobacillus,sphingomonas,and an increase in the abundance of enterobacteriaceae,suggesting that this may be an important cause of the increased incidence of urinary tract infections in postmenopausal women.It provides ideas for diagnosing urinary tract infections and non-antibiotic means for the treatment of urinary tract infections.
作者
李霞
王艳
郝莎莎
李柯桢
Li Xia;Wang Yan;Hao Shasha;Li Kezhen(Intensive Care Unit,Affiliated Hospital of Qingdao University,Qingdao 266003,China;Department of Nephrology,Affiliated Hospital of Qingdao University,Qingdao 266003,China;Department of Nephrology,Central Hospital,Luoyang 471009,China;Department of Nephrology,the Third People's Hospital of Qingdao City,Qingdao 266003,China)
出处
《国际泌尿系统杂志》
2020年第1期60-64,共5页
International Journal of Urology and Nephrology
关键词
泌尿道感染
微生物群
绝经期
生育力
Urinary Tract Infections
Microbiota
Menopause
Fertility