摘要
目的:研究牛磺酸对抑郁的干预作用。方法:采用行为绝望和利血平诱导的急性抑郁症两种小鼠模型。将模型小鼠随机分成6组:阴性对照组、模型对照组、氟西汀对照组、3个牛磺酸剂量组(0.5、1、2 g/kg mb),每组10只。通过悬尾实验、强迫游泳实验、旷场实验建立的行为绝望小鼠模型进行研究,研究牛磺酸对小鼠到达行为绝望时间的影响;通过分析眼睑下垂、肛温、出圈率等行为学指标,血清中促肾上腺皮质激素(adreno-corticotropic hormone,ACTH)、脑源性神经营养因子(brain derived neurotrophic factor,BDNF)、皮质醇(cortisol,COR),脑组织中5-羟色胺(5-hydroxytryptamine,5-HT)、去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)、单胺氧化酶(monoamine oxidase,MAO)、促肾上腺皮质激素释放因子(corticotropinreleasing factor,CRF)等生化指标,研究牛磺酸对急性抑郁的干预作用。结果:牛磺酸显著性缩短了行为绝望模型小鼠悬尾不动时间(P<0.01)和强迫小鼠游泳不动时间(P<0.01),在旷场实验中,显著性升高水平、垂直运动次数(P<0.01),眼睑下垂度无显著性差异(P>0.05),显著升高抑郁小鼠脑组织5-HT、DA、NE水平(P<0.05),降低MAO活性(P<0.01),并升高BDNF含量(P>0.05),同时抑制CRF、ACTH、COR的过度分泌(P<0.01),并改善相关生化指标。结论:牛磺酸可能是通过改善模型动物的激素分泌,干预利血平诱导的急性抑郁症。
Objective:To study the effect of taurine on depression-related behaviors and biochemical indexes in mouse models of depression.Methods:Two mouse models of behavioral despair or acute depression induced by reserpine,respectively were used for this study.The model mice were randomly divided into 6 groups:negative control,model control,fluoxetine control,and three taurine dose groups(0.5,1 and 2 g/kg mb),with 10 animals in each group.The effect of taurine on the despair time of mice was investigated by tail suspension,and forced swimming and open field tests,and its effect on depression-related behaviors such as ptosis,rectal temperature and escape rate as well as depression-related biochemical indicators including(adreno-corticotropic hormone)ATCH,brain derived neurotrophic factor(BDNF)and cortisol(COR)in serum and 5-hydroxytryptamine(5-HT),norepinephrine(NE),dopamine(DA),monoamine oxidase(MAO)and corticotropin-releasing factor(CRF)in brain tissue was evaluated in the mouse model of acute depression.Results:Taurine significantly shortened the suspension time of the behavioral despair model mice(P<0.01)and forced swimming time(P<0.01).In the open field test,taurine significantly increased the level and number of vertical movements(P<0.01),but resulted in no significant difference in ptosis rate(P>0.05).Meanwhile,taurine significantly increased the levels of 5-HT,DA and NE in brain tissue of depressed mice(P<0.05),decreased MAO activity(P<0.01),augmented BDNF content(P>0.05),inhibited excessive secretion of CRF,ACTH and COR(P<0.01),and improved other relevant biochemical indicators.Conclusion:Taurine may be an effective interventional strategy for reserpine-induced acute depression by improving hormone secretion in model animals.
作者
袁静
闫晨静
周茜
牛佳卉
吴梦颖
王亚旭
王颉
赵文
YUAN Jing;YAN Chenjing;ZHOU Qian;NIU Jiahui;WU Mengying;WANG Yaxu;WANG Jie;ZHAO Wen(Hebei Agricultural Products Processing Engineering Technology Center,College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第3期138-143,共6页
Food Science
基金
“十三五”国家重点研发计划重点专项（2018YFD0901004）
河北省现代农业产业技术体系生猪创新团队资助项目（HBCT2018110205）
河北省食品科学与工程学科“双一流”建设资金项目（2016SPGCA18）
