摘要
目的研究枫杨的化学成分及其抑制α-葡萄糖苷酶的活性。方法采用反复硅胶柱层析、葡聚糖凝胶(Sephadex LH-20)柱色谱、重结晶等分离纯化方法从枫杨乙酸乙酯部位分离单体化合物,依据主要理化性质和波谱数据鉴定化合物结构,运用96孔板测定化合物体外抑制α-葡萄糖苷酶活性。结果从乙酸乙酯部位分离鉴定了11个化合物,分别为β-胡萝卜苷(1)、水杨酸(2)、槲皮苷(3)、对香豆酸(4)、槲皮素(5)、5-羟基-2-甲氧基-1,4-萘醌(6)、β-谷甾醇(7)、没食子酸(8)、山柰酚(9)、槲皮素-3-O-β-D-半乳糖苷(10)、原儿茶酸(11)。体外α-葡萄糖苷酶抑制活性实验结果表明:在浓度为1mg·mL^-1时,化合物5、9对α-葡萄糖苷酶的抑制率超过90%,与阿卡波糖相当;化合物2、3、10对α-葡萄糖苷酶的抑制活性一般(约40%)。结论化合物2~4和9~11为首次从枫杨属植物中分离得到,化合物5、9对α-葡萄糖苷酶具有较强的抑制活性。
Objective To determine the chemical constituents of Pterocarya stenoptera and inhibition onα-glucosidase inhibitory activities.Methods The compounds were isolated and purified by column chromatography on silica gel,Sephadex LH-20 CC.The in vitro inhibition of each monomeric onα-glucosidase was evaluated by 96-well microtiter plate.The structures were elucidated based on the physicochemical properties and spectral datum.Results Eleven compounds were isolated and identified from Pterocarya stenoptera:daucosterol(1),salicylic acid(2),quercitrin(3),p-coumaric acid(4),quercetin(5),5-hydroxy-2-methoxy-1,4-naphthoquinone(6),β-sitosterol(7),gallic acid(8),kaempferol(9),quercetin-3-O-β-Dgalactopyranoside(10),and protocatechuic acid(11).The in vitro inhibition onα-glucosidase showed that the inhibition rate of compound 5 and 9 exceeded 90%at 1 mg·mL^-1,which was similar to acarbose.Conclusion Compounds 2-4,9-11 have been isolated from Pterocarya stenoptera for the first time.Compound 5 and 9 show strong inhibition onα-glucosidase.
作者
张远芳
谢朋飞
刘思妤
刘静
王俊杰
ZHANG Yuan-fang;XIE Peng-fei;LIU Si-yu;LIU Jing;WANG Jun-jie(Xiangnan College,Chenzhou Hunan 423000;Department of Pharmacy,First People's Hospital of Chenzhou,Chenzhou Hunan 423000)
出处
《中南药学》
CAS
2019年第12期2077-2080,共4页
Central South Pharmacy
基金
郴州市科技计划项目（No.CZ2015009）
关键词
枫杨
化学成分
α-葡萄糖苷酶抑制活性
Pterocarya stenoptera
chemical constituent
α-glucosidase inhibitory activity
