摘要
茚虫威对鳞翅目害虫幼虫具有卓越的杀虫活性,是替代传统杀虫剂及治理抗药性害虫的理想药剂。为了明确茚虫威对草地贪夜蛾的生物活性及对其主要解毒酶活性的影响,为使用茚虫威科学防治草地贪夜蛾提供参考,本研究采用浸叶法测定了广西南宁草地贪夜蛾种群3龄幼虫对茚虫威敏感性及增效醚(PBO)、磷酸三苯酯(TPP)、顺丁烯二酸二乙酯(DEM)对茚虫威的增效作用;并测定了亚致死浓度(LC 20)茚虫威对草地贪夜蛾体内MFO、GSTs和CarE酶的诱导作用。结果表明,茚虫威对草地贪夜蛾的LC 20、LC 50和LC 90分别为8.95、20.62 mg/L和73.57 mg/L。DEM、PBO和TPP对茚虫威的增效倍数依次为2.24、2.05和0.50倍。亚致死浓度(LC 20)茚虫威处理后对3龄幼虫体内GSTs的活性和MFO含量与无药剂处理(CK)相比显著升高(P<0.05),而CarE活性无显著变化(P>0.05)。本文结果显示,广西草地贪夜蛾仍可以用推荐剂量80 mg/L茚虫威防治,而代谢抑制剂PBO和DEM对茚虫威有明显的增效作用,且GSTs活性和MFO含量在茚虫威诱导后显著升高,初步推测这两种解毒酶可能影响将来草地贪夜蛾对茚虫威的抗药性发展。
Indoxacarb has excellent insecticidal activity against the larvae of lepidoptera pests.It is an ideal agent to replace traditional insecticides and control resistance pest.To provide guideline for its scientific application,the biological activity of indoxacarb and its induction to the detoxifying enzymes of Spodoptera frugiperda were investigated in this study.The sensitivity of 3rd instar larvae to indoxacarb and the synergistic effects of PBO,TPP and DEM to indoxacarb were determined by leaf dipping method.In addition,the induction of MFO,GSTs and CarE enzyme by LC 20 of indoxacarb was determined.The results showed that the LC 20,LC 50 and LC 90 values of indoxacarb were 8.95,20.62 mg/L and 73.57 mg/L,respectively.The synergistic ratios of DEM,PBO and TPP to indoxacarb were 2.24,2.05 and 0.50,respectively.Treatment with LC 20 of indoxacarb significantly increased the GSTs activity and MFO content in the 3rd instar larva compared with that of untreated control(CK)(P<0.05),but showed no effect on the CarE activity(P>0.05).These results suggested that the indoxacarb could still be used to prevent and control the S.frugiperda at the recommended dose of 80 mg/L,while the metabolic inhibitors PBO and DEM had obvious synergistic effects on indoxacarb,and GSTs activity and MFO content were significantly induced by LC 20 of indoxacarb.It was preliminarily speculated that these two enzymes might be involved in the development of indoxacarb resistance in future.
作者
王芹芹
崔丽
王立
黄伟玲
代黎明
袁会珠
芮昌辉
WANG Qinqin;CUI Li;WANG Li;HUANG Weiling;DAI Liming;YUAN Huizhu;RUI Changhui(Key Laboratory of Integrated Pest Management in Crops,Ministry of Agriculture and Rural Affairs,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Jining Normal University,Wulanchabu 012000,China)
出处
《植物保护》
CAS
CSCD
北大核心
2020年第1期78-81,共4页
Plant Protection
基金
中央级公益性科研院所基本科研业务费专项(Y2019YJ06)
国家重点研发计划(2016YFD0200504)
关键词
草地贪夜蛾
茚虫威
代谢抑制剂
增效作用
解毒酶
Spodoptera frugiperda
indoxacarb
metabolic inhibitor
synergistic ratios
detoxification enzyme
