摘要
目的研究幼年期、成年期和老年期小鼠侧脑室下区(subventricular zone,SVZ)中基因表达的增龄性变化。方法分别提取3周龄、6月龄和20月龄C57小鼠SVZ的mRNA,基于转录组测序及实时荧光定量PCR技术,检测SVZ中在不同年龄阶段的基因表达差异。结果以幼年期小鼠为对照,成年期检测到253个差异表达的基因,老年期检测到519个,以成年期作对照,老年期检测到147个差异表达的基因。GO分析发现成年期小鼠与幼年期相比差异表达基因主要涉及营养和代谢,老年期小鼠与成年期相比主要涉及中枢神经系统神经元分化等。KEGG分析发现差异表达基因主要涉及通路为蛋白质消化吸收、神经活性配体-受体相互作用等。结论该研究提供了不同年龄段SVZ转录水平基因变化的数据,为探讨SVZ的功能发育和退化,进一步治疗和抵抗神经衰老提供新的理论依据和方法。
Objective To study the age-related changes in SVZ gene expression of 3-week-old,6-month-old,and20-month-old mice.Methods RNA was extracted from the SVZ of mice at the three ages.The difference in gene expression of SVZ at the different ages was detected by RNA sequencing and quantitative real-time PCR.Results Compared with the levels in young mice,253 differentially expressed genes were detected in adult mice and 519 in old mice.Compared with the levels in adult mice,147 differentially expressed genes were detected in old mice.GO analysis found that the differentially expressed genes are mainly involved in nutrition and metabolism in adult mice compared with young,and central nervous system neuron differentiation among others in old mice compared with adult mice.KEGG analysis found that the differentially expressed genes are mainly involved in protein digestion and absorption,and neuroactive ligand–receptor interaction,among others.The differentially expressed genes were analyzed by GO and KEGG pathways analyses.Conclusions This study provides basic data on the changes of gene transcription levels with age in the SVZ,and also provides a new theoretical basis for treatment and resistance to nerve aging in the future.
作者
黄艺滢
石桂英
吕颖
李珂雅
白琳
HUANG Yiying;SHI Guiying;LYU Ying;LI Keya;BAI Lin(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS),Comparative Medicine Center,Peking Union Medical College(PUMC),Key Laboratory of Human Disease Comparative Medicine,National Health Commission of the People’s Republic of China,Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine,Beijing 100021,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第1期57-67,共11页
Chinese Journal of Comparative Medicine
基金
国家自然基金面上项目(31672374)
中国医学科学院医学与健康科技创新工程经费(2017-I2M-2-005)
