摘要
中国古老月季为月季育种提供了珍贵的基因资源。本研究利用RAPD (random amplified polymorphic DNA)标记分析22个中国古老月季的遗传多样性,明确了它们之间的亲缘关系,为现代月季育种工作提供了理论依据。40个RAPD引物在22个中国古老月季中共扩增出321个条带,其中301个为多态性条带,占93.77%,平均每个引物产生多态性条带7.525个。应用NTSYSpc 2.10e软件进行遗传一致度和遗传距离分析后用UPGMA方法进行聚类分析。结果表明,22个中国古老月季之间的遗传一致度在0.465 1~0.930 2之间,平均为0.657 8;遗传距离为0.071 7~0.812 6,平均为0.402 5。在遗传距离为0.627 9时可将22个中国古老月季划分成两大类。第一大类包括‘大富贵’和‘青莲学士’;第二大类包括:‘四面镜’‘月月红’‘绿萼’‘紫红香’‘金粉莲’‘金欧泛绿’‘春水绿波’‘桔囊’‘云蒸霞蔚’‘紫香绒’‘紫燕飞舞’‘思春’‘羽仕妆’‘朱衣一品’‘湖中月’‘玉玲珑’‘葡萄红’‘月月粉’‘软香红’‘赤胆红心’20个。在遗传距离最小的0.071 7处,有‘月月红’和‘绿萼’两个品种,其RAPD分子标记相似性达到了93.02%。本研究中分析的22个中国古老月季间表现出丰富的遗传多样性。
Chinese old garden roses can be used as precious genetic resources for rose breeding in the world.In this study,the genetic diversity of 22 Chinese old garden roses was analyzed by RAPD(random amplified polymorphic DNA)molecular markers,and their genetic relationships were clarified,which provided theoretical basis for the breeding work in the modern roses.A total of 321 bands are amplified from 40 RAPD primers,of which 301 were polymorphic bands,accounting for 93.77%,with 7.525 polymorphic bands per primer.Genetic identity and genetic distance were calculated by NTSYSpc 2.10e software and cluster analysis was analyzed by UPGMA.The results showed that the genetic identity of 22 Chinese old garden roses was 0.4651~0.9302,with an average of 0.6578.The genetic distance was 0.0717~0.8126,with an average of 0.4025.When the genetic distance was 0.6279,the 22 Chinese old garden roses in this study can be divided into two groups.The first major category included‘Dafugui’and‘Qinglianxueshi’,while the second major category including‘Simianjing’,‘Yueyuehong’,‘Lüe’,‘Zihongxiang’,‘Jinfenlian’,‘Jinoufanlü’,‘Chunshuilübo’,‘Junang’,‘Yunzhengxiawei’,‘Zixiangrong’,‘Ziyanfeiwu’,‘Sichun’,‘Yushizhuang’,‘Zhuyiyipin’,‘Huzhongyue’,‘Yulinglong’,‘Putaohong’,‘Yueyuefen’,‘Ruanxianghong’and‘Chidanhongxin’varieties.When the genetic distance was 0.6279,the similarity of RAPD markers for‘Yueyuehong’and‘Lüe’can reach 93.02%,it was shown various genetic diversity of 22 Chinese old garden roses in our studies.
作者
李玉洁
庄得凤
赵成日
崔凯歌
Li Yujie;Zhuang Defeng;Zhao Chengri;Cui Kaige(Agriculture College of Yanbian University,Yanji,133002;Agricultural College,Inner Mongolia University for Nationalities,Tongliao,028000;Economic Crop Institution,Liaoning Academy of Agricultural Sciences,Liaoyang,111000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第24期8287-8294,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(31660319)资助
