摘要
【目的】植物通过启动一系列信号传导过程来应对外部环境,这些过程通常涉及多种蛋白激酶,包括钙调神经磷酸酶B样蛋白互作激酶(calcineurin B-like protein-interacting protein kinases, CIPKs)。为更加清晰全面地了解水稻CIPK基因家族,本研究根据最新的基因组测序数据对水稻基因组中的CIPKs进行了鉴定。【方法】通过探讨拟南芥和水稻中CIPKs蛋白家族的结构特点,结合生物信息学和qRT-PCR技术系统分析水稻中CIPKs家族蛋白的结构。结合转录组数据,比较了粳稻云引受稻瘟病菌诱导后的表达情况。【结果】根据最新的水稻基因组数据,鉴定出31个水稻OsCIPK基因。系统发育树分析结果表明,31个OsCIPK基因可分为5个亚家族,这些亚家族具有不同的外显子-内含子和UTR的结构特点。从广谱抗稻瘟病品种粳稻云引受稻瘟病菌诱导的基因表达谱的趋势聚类中筛选出了OsCIPK5基因并对其进行了表达分析,结果表明,云引中OsCIPK5基因受稻瘟病菌的诱导表达。【结论】内含子缺失和片段重复在水稻OsCIPK基因家族的扩展中起到重要作用,同时OsCIPK5受到稻瘟病菌的诱导表达。
【Objective】 The family of calcineurin B-like protein-interacting protein kinase genes(CIPKs) in rice was studied using the latest sequencing data to further understand the signal transduction involving a variety of kinases in plants in respond to environmental changes. 【Methods】 The structures of CIPKs in Arabidopsis thaliana and rice were analyzed. Combining the bioinformatics and qRT-PCR technology, expressions of the CIPK familie and that of the genes induced by M. grisea in Japonica rice cv. ’Yunyin’ were compared. 【 Results】 A total of 31 OsCIPK genes were identified in the rice genome databank. The phylogenetic tree analysis showed that these 31 OsCIPKs could be divided into 5 subfamilies, which had different structural characteristics of exon-intron and UTR. The expression of OsCIPK5, which was selected from a trend cluster of the gene expressiong profile of ’Yunyin’ induced by M. grisea, could be induced by M. grisea. 【Conclusions】 It appeared that the intron deletion and fragment duplication played an important role in the expansion of OsCIPK family in rice,and that OsCIPK5 expression was induced in ’Yunyin’ by M. grisea.
作者
喻丝丝
罗曦
连玲
许惠滨
陈丽萍
魏毅东
蔡秋华
谢华安
张建福
YU Si-si;LUO Xi;LIAN Ling;XU Hui-bin;CHEN Li-ping;WEI Yi-dong;CAI Qiu-hua;XIE Hua-an;ZHANG Jian-fu(College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350019,China;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture/Fuzhou Branch,National Rice Improvement Center/Incubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences&Technology/Base of South-China,National Key Laboratory of Hybrid Rice/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/National Engineering Laboratory of Rice,Fuzhou,Fujian 350003,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第11期1237-1245,共9页
Fujian Journal of Agricultural Sciences
基金
国家重点研发计划项目(2016YFD0300508)
福建省科技计划公益类专项(2018R1021-5)
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-1-1)
