摘要
目的研究吴茱萸挥发油对小鼠的急性毒性作用。方法采用多浓度试验进行吴茱萸挥发油急性毒性研究,不同浓度的挥发油提取液进行灌胃,灌胃体积按25 mL/kg计算,1次/d,连续14d给药。给药后对小鼠急性毒性症状进行观察,记录小鼠体质量变化情况,通过测定挥发油LD50值来评价吴茱萸挥发油急性毒性。结果灌服低浓度的挥发油后,小鼠死亡率低,但出现怠动、活动减少、安静等症状;灌服高浓度挥发油后小鼠全部死亡,同时观察到小鼠出现呼吸抑制、腹泻等症状。计算得出吴茱萸挥发油LD5。值为1.708 mL/(kg・d),95%可信限为1.496〜1.899mL/(kg・d)。结论吴茱萸挥发油具有急性毒性。
Objective Study on the acute toxicity of volatile oil from Evodia Rutaecarpa in mice.Methods Acute toxicity of volatile oil of Evodia Rutaecarpa was studied by multi-concentration test.The volume of volatile oil extract with different concentrations was calculated by 25 mL/kg,which was administered once a day for 14 consecutive days.Evaluation on the acute toxicity of volatile oil from Evodia Rutaecarpa by observing acute toxicity of symptoms in mice,recording the weight changes of the mice and determing the LD50.Results After the administration of low concentration of volatile oil,the mortality of the mice was low,but all the mice showed signs of idling,decreased activity and quiescence.All the mice died after high concentration of volatile oil was administered,and respiratory inhibition and diarrhea were observed.LD50 was 1.708 mL/(kg-d)at the volatile oil from Evodia Rutaecarpa,with 95%confidence limit was 1.496・1.899 mL/(kg-d).Conclusion The volatile oil from Evodia Rutaecarpa had toxicity.
作者
李明
任晓静
张逊
冯昊
袁金斌
LI Ming;REN Xiaojing;ZHANG Xun;FENG Hao;YUAN Jinbin(Pharmacy Department of Tangshan People's Hospital,Tangshan 063000,Hebei Province,China;Pharmacy Department of Tangshan Union Hospital,Tangshan 063000,Hebei Province,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)
出处
《吉林中医药》
2019年第12期1642-1644,共3页
Jilin Journal of Chinese Medicine
基金
河北省中医药管理局课题（2018336）
关键词
吴茱萸
小鼠
急性毒性
Evodia Rutaecarpa
mice
acute toxicity
