摘要
利用Bliss模型,研究不同含量复配的3种脂质伴随物α-生育酚、γ-谷维素和植物甾醇在乙酸乙酯非极性介质中清除DPPH自由基能力和相互作用类型,采用傅里叶变换红外光谱初步阐释相互作用机理。结果表明:3种脂质伴随物单独清除DPPH自由基能力强弱为α-生育酚>γ-谷维素>植物甾醇;3种脂质伴随物两两复配后清除DPPH自由基主要呈现拮抗作用。动力学研究发现,非极性介质复配体系清除DPPH自由基的反应速率快慢由体系中主抗氧化剂决定;采用傅里叶变换红外光谱仪检测复配脂质伴随物光谱,发现羟基右移,推测氢键的形成可能是导致3种脂质伴随物在乙酸乙酯中产生拮抗作用的原因。
Bliss model was used to study the capacity of scavenging DPPH free radical and interactions in different contents ofα-tocopherol,γ-oryzanol and phytosterol complex in ethyl acetate non-polar medium,and Fourier transform infrared spectroscopy was used to interpret the interaction mechanism.The results showed that the capacity ofα-tocopherol on scavenging DPPH free radical was the strongest,followed byγ-oryzanol and phytosterol.The interaction of scavenging free radicals of the three lipid concomitants after two-two complex was mainly antagonistic.The kinetic study showed that the reaction rate of mixture system for scavenging DPPH free radicals in the non-polar medium was mainly determined by the main antioxidant.Then the hydroxyl bond was found to shift to the right by Fourier transform infrared spectroscopy,surmising the formation of hydrogen bonds was a possible cause of the antagonism of the three lipid concomitants in ethyl acetate.
作者
张莉莎
倪菁潞
刘睿杰
常明
金青哲
王兴国
ZHANG Lisha;NI Jinglu;LIU Ruijie;CHANG Ming;JIN Qingzhe;WANG Xingguo(Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,National Engineering Technology Research Center of Functional Food,College of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2019年第12期104-108,130,共6页
China Oils and Fats
基金
国家自然科学基金面上项目(31872895)
