摘要
为筛选白鲜愈伤组织培养的适宜培养基,试验以延边地区野生白鲜种子为材料,研究了MS培养基中植物生长调节剂种类及其浓度对愈伤组织诱导和生长以及愈伤组织中白鲜碱、黄柏酮和梣酮积累的影响。结果表明:在MS+2.0 mg/L NAA+2.0 mg/L 6-BA以及MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA培养基中,愈伤组织诱导率及生长较好;在MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA培养的愈伤组织中,白鲜碱和梣酮的有效积累量最高,在MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA培养的愈伤组织中,黄柏酮的有效积累量最高。
In orther to screen the suitable medium for callus culture,seeds of wild Dictamnus dasycarpus growing in Yanbian area were used as materials in this experiment,the effects of plant growth regulators and their concentrations in MS medium on inducing and growth of callus,and accumulation of dictamnine,obacunone and fraxinellone in callus were investigated.The results showed that the callus grew well in the MS+2.0 mg/L NAA+2.0 mg/L 6-BA,and MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA medium.The effective accumulation of dictamnine and fraxinellone in callus on the MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA medium,and obacunone in callus on the MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA medium were the highest.
作者
王馨琪
贾晓龙
刘卉
李美阳
南桂仙
WAGN Xinqi;JIA Xiaolong;LIU Hui;LI Meiyang;NAN Guixian(Agricultural College of Yanbian University,Yanji Jilin 133002,China)
出处
《延边大学农学学报》
2019年第4期55-61,共7页
Agricultural Science Journal of Yanbian University
基金
吉林省教育厅“十三五”科学技术研究项目(吉教科合字[2016]第254号)
延边大学应用基础项目(延大科合字2019第16号)
关键词
白鲜
愈伤组织
白鲜碱
黄柏酮
梣酮
Dictamnus dasycarpus
callus
dictamnine
obacunone
fraxinellone
