摘要
目的:研究甘青铁线莲皂苷(CTS)对大鼠心肌缺血/再灌注(MI/R)损伤的影响。方法:可逆性冠脉左前降支结扎缺血30 min再灌注3 h复制MI/R模型,将SD大鼠随机分为假手术组、模型组、CTS低、中、高剂量组(50,100,200 mg·kg^-1),每组10只。采用伊文思蓝(EB)、2,3,5-氯化三苯基四氮唑蓝(TTC)双染法测定心肌梗死面积,苏木精-伊红(HE)染色法观察心肌病理学形态变化,并检测血清中乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、超氧化物歧化酶(SOD)及丙二醛(MDA)水平。结果:与模型组比较,中、高剂量的CTS可明显缩小心肌梗死面积,并显著降低血清中LDH、CK-MB、TNF-α、IL-6及MDA的水平,显著增加血清中SOD的活性(P<0.05或P<0.01),且CTS各剂量组心肌组织的病理损伤也低于模型组。结论:CTS对大鼠MI/R损伤具有保护作用,其机制可能与其抑制炎症因子的释放和调节氧化应激有关。
Objective:To investigate the protective effects of total saponins extracted from Clematis tangutica(CTS)on myocardial ischemia/reperfusion injury in rats.Methods:An in vivo model of myocardial ischemia 30 min followed by 3 h reperfusion was made by reversibly ligating coronary left descending artery.SD rats were randomly divided into the sham-operated group,the model group,CTS low,medium and high dose groups(50,100 and 200 mg·kg^-1)with 10 rats in each group.The myocardial infarct size was measured by double staining of evans blue(EB)and 2,3,5-triphenyltetrazolium chloride(TTC),the pathological changes of myocardium were observed by hematoxylin-eosin(HE)staining,and the levels of lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),superoxide dismutase(SOD)and malondialdehyde(MDA)in serum were detected.Results:Compared with the model group,medium and high dose CTS groups significantly reduced the infarct size,decreased the levels of LDH,CK-MB,TNF-α,IL-6 and MDA in serum,and increased the activity of SOD(P<0.05 or P<0.01).In addition,the pathological damage of myocardium in CTS groups was also slighter than that in the model group.Conclusion:CTS has cardioprotective effect against MI/R injury,and the mechanism may be related to its effects of inhibiting the release of inflammatory cytokines and regulating oxidative stress.
作者
姚敏娜
牟菲
张伟
李韦韦
殷英
杨志福
文爱东
李锐莉
Yao Minna;Mou Fei;Zhang Wei;Li Weiwei;Yin Ying;Yang Zhifu;Wen Aidong;Li Ruili(Department of Pharmacy,Xijing Hospital,Air Force Military Medical University,Xi'an 710032,China)
出处
《中国药师》
CAS
2019年第12期2173-2176,共4页
China Pharmacist
基金
国家自然科学基金项目(编号:81603320)
关键词
甘青铁线莲皂苷
心肌缺血/再灌注损伤
炎症
氧化应激
Total saponins from Clematis tangutica
Myocardial ischemia/reperfusion injury
Inflammation
Oxidative stress
