摘要
目的建立高效液相色谱法同时测定川归补肾通络胶囊中丹酚酸B和丹参酮ⅡA 2种成分的含量。方法采用Agilent ZORBAX SB-C18色谱柱(5μm,4.6 mm×250 mm);乙腈-0.1%磷酸溶液为流动相,梯度洗脱,体积流速为1.0 m L/min;检测波长为270 nm(丹参酮ⅡA)、286 nm(丹酚酸B);柱温设定为20℃。结果丹酚酸B、丹参酮ⅡA进样浓度分别在44.83~448.3μg/mL、0.96~9.60μg/mL范围内线性关系均良好,回归方程分别为Y=12 650X-1973.3(r=0.9996)、Y=54 274X+22 206(r=0.997),平均加样回收率分别为99.7%(RSD=1.9%)、97.4%(RSD=1.7%)。结论方法操作简便、结果准确、重复性及稳定性均较好,可用于川归补肾通络胶囊的质量控制。
Objective To establish a high performance liquid chromatography(HPLC) method for simultaneous determination of salvianolic acid B and tanshinone ⅡA in Chuangui Bushen Tongluo Capsules. Methods The content of sal vianolic acid B and tanshinone ⅡA was determined by HPLC. Agilent ZORBAX SB-C18(5 μm, 4.6 mm×250 mm) was used as the column. Acetonitrile(A)-0.1% aqueous phosphoric acid(B) was used as the mobile phase with gradient elution.With the detection wavelength of 270 nm tanshinone ⅡA and 286 nm vianolic acid B. The flow rate was 1.0 mL/min and column temperature was 20℃. Results The linear ranges of salvianolic acid B and tanshinone Ⅱ A were 44.83-448.3 μg/mL, 0.96-9.60 μg/mL, respectively. The regression equation were Y = 12 650 X-1973.3(r = 0.9996),Y =54 274 X+22 206(r = 0.997), respectively. The average recoveries were 99.7%(RSD = 1.9%), 97.4%(RSD = 1.7%),respectively. Conclusion The established method has the advantages of simple operation, strong specificity and good reproducibility, which can be used to control the quality of Chuangui Bushen Tongluo Capsules.
作者
余静
朱应成
祁俊
梁朝锋
徐斌
YU Jing;ZHU Yingcheng;QI Jun;LIANG Chaofeng;XU Bin(Preparation Room,Wuhu Hospital of Traditional Chinese Medicine,Anhui Province,Wuhu241000,China)
出处
《中国医药导报》
CAS
2020年第1期25-28,共4页
China Medical Herald
基金
安徽省卫生计生委中医药科研课题项目（2016zy41）
安徽省芜湖市科技计划项目（2015hm09）
关键词
川归补肾通络胶囊
丹酚酸B
丹参酮ⅡA
高效液相色谱法
Chuangui Bushen Tongluo Capsules
Salvianolic acid B
Tanshinone ⅡA
High performance liquid chromatography
