摘要
目的制备负载CoFe2O4@MnFe2O4磁性纳米粒子(简称:Co@Mn MNPs)与二氢卟吩e6(简称:Ce6)的多功能纳米胶囊(简称:MNCPs),研究MNCPs对胃癌的靶向性磁共振成像与光动力治疗效果。方法采用微乳液法制备双亲性高分子聚异丁烯-铝-马来酸酐(简称:PMA)微泡包载Co@Mn MNPs与Ce6的纳米胶囊,在其表面偶联靶向分子叶酸,形成PMA-Co@Mn/Ce6-FA MNPs,制备出具有靶向性诊断与治疗的多功能纳米胶囊。检测MNCPs的表征了解其特征,并利用细胞实验探测细胞对MNCPs的利用,另外利用胃癌荷瘤裸鼠研究MNCPs在小鼠体内的磁共振成像与靶向性治疗效果。结果MNCPs粒径约(150±15)nm,呈单分散的球形,包封率为(97%),包载量约(3.1%)。细胞实验证明肿瘤细胞对MNCPs有较好的摄取,并且摄入的MNCPs对肿瘤细胞的毒性极小,还可以在633nm激光照射下对肿瘤细胞产生良好的光动力治疗(PDT)效果。动物实验证明MNCPs能增强小鼠体内肿瘤部位的磁共振成像效果,对胃癌具有显著的靶向性光动力治疗效果。结论多功能纳米胶囊对胃癌具有靶向性磁共振诊断与光动力治疗作用。
Objective To prepare multifunctional magnetic nanocapsules(MNCPs)co-loaded Co@Mn MNPs magnetic nanoparticles and photosensitizer chlorin e6(Ce6)for targeted resonance imaging(MRI)and photodynamic therapy. Methods MNCPs were synthesized by a microemulsion method to co-load Co@Mn MNPs and Ce6 by PMA,and then conjugated a target molecules(folic acid,FA)to fabricate PMA-Co@Mn/Ce6-FA MNPs(Abb:MNCPs). The biologic application of MNCPs was investigated by in vitro study. In vivo MRI and photodynamic therapy study were achieved in gastric cancer tumor-bearing nude mice. Results The characterization showed that the MNCPs exhibited an excellent dispersibility and stability with morphology of spherical capsules.The average size of MNCPs was about(150 ±15)nm and encapsulation efficiency(EE%)and % loading of Ce6 in approximately were up to(97%)and(3.1%). The cell experiment demonstrated that MNCPs showed the good biocompatibility,and possessing favorable photodynamic therapy(PDT)effect. In vivo experiment study indicated that nanocapusles exhibited an outstanding targeted magnetic resonance imaging and photodynamic therapy.Conclusion The nanocapsules can acquire the enhanced MR imaging and photodynamic therapy.
作者
张玉会
张倩
章阿敏
潘少君
洪立新
何井华
ZHANG Yuhui;ZHANG Qian;ZHANG A⁃min;PAN Shaojun;HONG Lixin;HE Jing⁃hua(Zhujiang Hospital,Southern Medical University,Guangzhou 510280,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第24期3752-3759,共8页
The Journal of Practical Medicine
基金
上海青年科技英才扬帆计划资助(编号：19YF1422300)
关键词
磁性纳米粒子
共负载磁性纳米胶囊
荧光标记
光动力治疗
磁共振成像
magnetic nanoparticles
Co-loaded magnetic nanocapsules
fluorescent labeling
photodynamic therapy
magnetic resonance imaging
