摘要
目的利用siRNA沉默子宫内膜癌lshikawa细胞中p110β的表达,探讨其对子宫内膜癌细胞凋亡与侵袭的影响。方法构建p110βsiRNA的表达载体,并转染Ishikawa细胞。RT-PCR方法检测转染前后Ishikawa细胞中p110β的表达情况;流式细胞技术分析转染前后Ishikawa细胞的凋亡变化,Transwell实验检测转染前后Ishikawa细胞侵袭能力的变化。结果 siRNA重组质粒pGCsi-p110β对Ishikawa细胞中p110βmRNA的表达抑制率为88.90%,明显高于对照组(P<0.05)。转染pGCsi-p110β的Ishikawa细胞凋亡率为18.3%,显著高于对照组的11.0%,而Ishikawa细胞穿膜数目显著低于对照组(P<0.05)。结论沉默p110β的表达诱导子宫内膜癌lshikawa细胞凋亡并抑制侵袭。
Objective To silence p110 beta in endometrial cancer lshikawa cells by siRNA and investigate its effect on apoptosis and invasion of endometrial cancer cells. Methods p110 beta siRNA expression vector was constructed and transfected into Ishikawa cells. RT-PCR was used to detect the expression of p110 beta in Ishikawa cells before and after transfection. Flow cytometry was used to analyze the apoptotic changes of Ishikawa cells. Transwell assay was used to detect the changes of invasive ability of Ishikawa cells. Results The inhibition rate of siRNA recombinant plasmid pGCsip110 beta on the expression of p110 beta in Ishikawa cells was 88.90%, significantly higher than that in the control group(P<0.05). The apoptotic rate of Ishikawa cells transfected with pGCsi-p110 beta was 18.3%, significantly higher than that in the control group(11.0%, P<0.05), but the number of Ishikawa cells transfected with pGCsi-p110 beta was significantly lower than that in the control group(P<0.05). Conclusion Silencing the expression of p110 beta could induce apoptosis and inhibit invasion of lshikawa cells.
作者
张琛
杨清
ZHANG Chen;YANG Qing(Department of gynaecology,shengjing hospital of china medical university,Shenyang 110004,China)
出处
《解剖科学进展》
2019年第6期688-690,共3页
Progress of Anatomical Sciences
基金
盛京自由研究者基金(201704)
