摘要
目的探索瞬时受体电位香草素受体4型通道蛋白(transient receptor potential vanilloid receptor 4,TRPV4)在骨关节炎(osteoarthritis,OA)软骨与正常软骨中的表达差异,为进一步研究其在OA防治中的作用提供理论依据。方法取膝关节OA患者软骨组织(OA组)与股骨颈骨折患者正常软骨组织(对照组)。其中,OA组男6例,女9例;年龄55~78岁,平均69岁;Kellgren-Lawrence(K-L)评分为(3.0±0.8)分。对照组男5例,女10例;年龄57~91岁,平均71岁。两组患者性别及年龄比较差异无统计学意义(P>0.05)。采用Western blot、实时荧光定量PCR、Masson染色以及免疫组织化学染色,观察并比较两组软骨组织中TRPV4蛋白及mRNA表达差异,以及软骨组织退变程度;分析OA组患者K-L评分与TRPV4阳性细胞率的相关性。结果OA组TRPV4蛋白及mRNA相对表达量分别为0.454±0.199、2.951±1.200,高于对照组的0.165±0.074、1.437±0.682,其中蛋白相对表达量组间差异有统计学意义(t=2.718,P=0.026)。组织染色显示,OA组软骨细胞排列紊乱,细胞外基质失去正常结构,局部软骨缺损可达深层,退变组织中可见较多TRPV4阳性细胞,阳性细胞率为37.353%±13.496%;对照组软骨细胞层次分明,阳性细胞率仅为9.642%±3.284%;两组阳性细胞率比较差异有统计学意义(t=7.491,P=0.000)。OA组TRPV4阳性细胞率与OA的K-L评分成正相关(r=0.775,P=0.001)。结论TRPV4在OA软骨组织中表达升高,且其表达升高与OA进展有相关性。
Objective To investigate the differential expression of transient receptor potential vanilloid receptor4(TRPV4) protein in the osteoarthritis(OA) and normal cartilages, and explore the role of TRPV4 in the prevention and treatment of OA. Methods The cartilage tissues from the patients of knee OA(OA group) and femoral neck fracture(control group) were taken. In OA group, there were 6 males and 9 females;the age ranged from 55 to 78 years(mean, 69 years);the Kellgren-Lawrence(K-L) score was 3.0±0.8. In control group, there were 5 males and 10 females;the age ranged from 57 to 91 years(mean, 71 years). There was no significant difference in gender and age between the two groups(P>0.05). Western blot, real-time fluorescence quantitative PCR, Masson staining, and immunohistochemical staining were used to detect the difference in protein and mRNA expressions of TRPV4 between the OA and normal cartilages.Then the relationship between the K-L score of OA and the rate of TRPV4-positive cells was analyzed. Results The relative expression of TRPV4 protein and mRNA in OA group were 0.454±0.199 and 2.951±1.200, which were higher than those in control group(0.165±0.074, 1.437±0.682). The difference in relative expression of TRPV4 protein was significant(t=2.718, P=0.026). Histology observation showed that the chondrocytes arranged disorderly in OA group, the structure of extracellular matrix was abnormal, and the cartilage defect reached the deep layer. There were more TRPV4-positive cells in the degenerated tissue, and the rate of TRPV4-positive cells was 37.353%±13.496%. The chondrocytes were arranged well in control group, and the rate of TRPV4-positive cells was only 9.642%±3.284%. There was a significant difference between the two groups(t=7.491, P=0.000). The rate of TRPV4-positive cells in OA group was positively correlated with the OA K-L score(r=0.775, P=0.001). Conclusion The TRPV4 expression increased in OA cartilages that may contribute to the development of OA.
作者
姚旺祥
戴晗豪
董佩龙
桂鉴超
YAO Wangxiang;DAI Hanhao;DONG Peilong;GUI Jianchao(Department of Sports Medicine and Joint Surgery,the Affiliated Nanjing Hospital of Nanjing Medical University,Nanjing Jiangsu,210000,P.R.China;Department of Orthopaedics,the Affiliated Hangzhou Hospital of Nanjing Medical University,Hangzhou Zhejiang,310006,P.R.China)
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2020年第1期63-68,共6页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金面上项目(81672210)~~
