摘要
脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)是粮食中常见的一种次生代谢产物,对人类和牲畜的健康有许多不良影响。该实验采用高效液相色谱法(high performance liquid chromatography, HPLC)检测DON的含量变化,探究了电化学氧化法(electrochemical oxidation,EO)对DON纯品以及玉米酒精糟(wet distillers grains with solubles broths,WDGS)中DON的降解效果。结果表明,DON降解率与处理时间及电压呈正相关,1.2 V电压处理DON溶液60 min,降解率可达76.4%,且反应遵从一级动力学反应过程。当NaCl添加量为0.2 mol/L时,可达最佳降解效果。此外,处理60 min后受污染的WDGS中DON的降解率为43.28%,加入电解质有利于提高降解率。玉米酒精糟中粗蛋白质和脂质含量在电解前后没有显著变化。因此,电化学氧化可作为发酵工业中去除发酵残渣中DON的有效方法。
Deoxynivalenol(DON) is a secondary metabolite commonly found in grains, which can lead to many adverse health effects to human and livestock. The degradation effects of electrochemical oxidation(EO) on pure DON and in wet distillers′ grains with soluble(WDGS)broth were investigated. The results showed that 76.4% of DON degraded within 60 min at a voltage of 1.2 V, and the degradation process could be expressed by first-order kinetics when the optimum NaCl concentration was 0.2 mol/L. In addition, the degradation rate of DON in contaminated WDGS was 43.28% after 60 min of treatment. Addition of electrolyte was favorable for the degradation. The crude protein and lipid content in WDGS did not change significantly before and after electrolysis. The electrochemical oxidation process can be used as an effective method for removing DON from WDGS in the corn fermentation industry.
作者
熊粟栗
袁文娟
高婧琪
李晓
李华志
张杰
XIONG Suli;YUAN Wenjuan;GAO Jingqi;LI Xiao;LI Huazhi;ZHANG Jie(College of Life Science,Key Laboratory of Bio-resource and Eco-environment of Ministry of Education,Sichuan University,Chengdu 610064,China;Sichuan Hongzhan Industry Group,Neijiang 641200,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第24期22-26,共5页
Food and Fermentation Industries
基金
四川省高新重点项目(2017FZ0071)
关键词
高效液相色谱法
脱氧雪腐镰刀菌烯醇
电化学氧化
湿态玉米酒精糟
一级动力学反应
high performance liquid chromatography(HPLC)
deoxynivalenol(DON)
electrochemical oxidation(EO)
wet distillers grains with solubles(WDGS)
first-order kinetic reaction
