摘要
分离鉴定地黄根腐病的病原菌,对其致病性和生物学特性进行研究。利用平板分离法对地黄根腐病的病原菌进行分离纯化;利用测序技术和遗传进化分析对所得菌株进行鉴定;利用回接试验对所得菌株进行致病性检测;利用生长监测,确定所得菌株的各项生理学特性。获得一株菌落橙黄色,边缘整齐,表面光滑、湿润菌体椭圆形,有疑似出芽现象的真菌;该菌与帕鲁迪根红酵母(Rhodotorula paludigena)簇集在一起,置信度为91,遗传距离最近,具有密切的亲缘关系;该酵母菌接种侵染20 d后,地黄块根根腐病发病率达到100%;该菌株最适生长温度为28℃,最适生长pH为5.0;该酵母菌株能耐受阿魏酸、香草酸和对羟基苯甲酸(与根腐病发病密切相关的地黄根部分泌物)的最高浓度为0.5 g/L。初步证实了Rhodotorula paludigena DH5能够引起地黄根腐病,且该病原在富含酚酸类化合物的地黄根际具较强的生态适应能力。
We aimed to isolate and identify the pathogens resulting in Rehmannia glutinosa root rot as well as study its pathogenicity and biological characteristics.In this study,streak plate method was used to isolate and purify the pathogens of rot root in R.glutinosa.ITS sequencing and genetic evolution analysis were used to identify the isolated strains.Inoculation experiment was used to confirm the pathogenic of the isolated potential pathogens.The growth test was performed to analyze physiological characteristics of the isolated stain.One of the obtained strains presented the colony morphology of orange color,the neat edge,the smooth and moist surface,and microscopic morphology with oval shape,suspected to be a fungus with buds.The strain was clustered together with the Rhodotorula paludigena at confidence of 91 and they displayed the close genetic relation.R.glutinosa roots inoculated with the strain and the incidence was 100%at day 20.The optimal growth temperature and pH of the strain was 28℃and 5.0,respectively.The strain grew well in the medium with 0.5 g/L ferulic acid,vanillic acid and p-hydroxybenzoic acid,i.e.,the secretion in the root of R.glutinosa that was closely related to the root rot of R.glutinosa.It is preliminarily verified that R.paludigena DH5 may cause the root rot of R.glutinosa and well adapts to the rhizosphere environment containing a large content of phenolic compounds.
作者
王亚利
康春晓
杨传臻
魏艺璇
王瑞飞
李明军
杨清香
WANG Ya-li;KANG Chun-xiao;YANG Chuan-zhen;WEI Yi-xuan;WANG Rui-fei;LI Ming-jun;YANG Qing-xiang(College of Life Sciences,Henan Normal University,Xinxiang 453007;Key Laboratory for Microorganisms and Functional Molecules(Henan Normal University),University of Henan Province,Xinxiang 453007;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province,Xinxiang 453007)
出处
《生物技术通报》
CAS
CSCD
北大核心
2020年第1期37-44,共8页
Biotechnology Bulletin
基金
国家自然科学基金项目(21277041,21477035,U1504301)
河南省高等学校重点科研项目(15A180016)
关键词
地黄
根腐病
酵母菌
酚酸
生物学特性
Rehamannia glutinosa
phytophthora root rot
Rhodotorula paludigena
phenolic acid
biological characteristics
