摘要
以鼠李糖乳杆菌为研究对象,利用电感耦合等离子发射光谱(ICP-OES)法测定鼠李糖乳杆菌的富硒量和富硒率,在单因素实验的基础上,应用响应面优化工艺条件,所得最佳工艺条件为:盐浓度9%、培养pH 6.5、培养温度37℃.经实验验证富硒率为65.35%,相对于未胁迫组,富硒量增加了437.63μg/g,富硒率提高了14.7%.
Taking Lactobacillus rhamnosus as the research object,the selenium content and selenium enrichment rate of Lactobacillus rhamnosus were measured by inductively coupled plasma emission spectrometry(ICP-OES).On the basis of single factor experiment,response surface optimization was applied to optimize the process conditions.The optimum process was as follows:salt concentration 9%,culture pH 6.5 and culture temperature 37℃.The selenium enrichment rate was 65.35%,which increased by 437.63μg/g and 14.7%compared with the unstressed group.
作者
徐颖
杨玉洁
何成艳
贺佳璇
魏丽娜
邬淑芳
XU Ying;YANG Yu-jie;HE Cheng-yan;HE Jia-xuan;WEI Li-na;WU Shu-fang(School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)
出处
《陕西科技大学学报》
CAS
2020年第1期58-63,共6页
Journal of Shaanxi University of Science & Technology
基金
陕西省科技厅农业科技攻关计划项目(2017TSCXL-NY-02-03)
陕西省教育厅专项科研计划项目(18JK0094)
陕西科技大学博士科研启动基金项目(2016BJ-21)
陕西省大学生创新创业训练计划项目(S201910708081)
关键词
鼠李糖乳杆菌
盐胁迫
电感耦合等离子发射光谱
响应面
Lactobacillus rhamnosus
salt stress
inductively coupled plasma emission spectroscopy
response surface
