摘要
目的探究miRNA-107在急性髓系白血病中的表达及意义。方法采用荧光实时定量PCR(qRT-PCR)法检测100例在本院初诊为原发急性髓系白血病患者(观察组)及同期行骨髓穿刺检查的非血液系统肿瘤疾病患者(对照组)骨髓单个核细胞miRNA-107的表达水平。采用直接测序法检测IDHl、IDH2、NPML、FLT3-ITD、DNMT3A和CEBPα突变。采用受试者工作曲线(ROC)分析miRNA-107对急性髓系白血病患病风险的预测效能。以miRNA-107中位表达水平为分界线将100例患者分为miRNA-107高表达组(50例)和miRNA-107低表达组(50例),分析两组患者miRNA-107表达与各临床指标、基因突变及预后的关系。结果观察组miRNA-107水平明显低于对照组(P<0.05),miRNA-107预测急性髓系白血病患病风险的ROC曲线下面积为0.877。miRNA-107高表达组患者HGB水平、NPM1野生型占比及完全缓解率均显著高于miRNA-107低表达组(P<0.05)。两组患者性别、年龄、WBC、PLT、骨髓原始细胞比例、CEBPα、FLT3-ITD、IDH1/2及DNMT3A等方面比较差异无统计学意义(P>0.05)。miRNA-107高表达组患者中位总生存(OS)时间及中位无复发生存(RFS)时间分别为26个月、21.5个月,均显著高于miRNA-107低表达组(15个月、11.8个月,P<0.05);Cox回归模型多因素分析显示低miRNA-107表达水平是影响急性髓系白血病患者预后不良的独立危险因素(P<0.05)。结论miRNA-107高表达是急性髓系白血病患者预后良好的标志物,miRNA-107可作为预测急性髓系白血病患病风险的指标,也可作为评估急性髓系白血病患者预后的重要指标。
Objective To explore the expression and significance of miRNA-107 in acute myeloid leukemia.Methods Real-time quantitative PCR(qRT-PCR)was used to detect 100 cases of bone marrow mononuclear cells from patients with primary acute myeloid leukemia(the observation group)and non-hematologic tumor disease(the control group)who underwent bone marrow puncture at the same time.With this,the expression level of cellular miRNA-107 were measured in both groups.The direct sequencing method was used to detect IDHl,IDH2,NPML,FLT3-ITD,DNMT3A and CEBPαmutations.The predictive efficacy of miRNA-107 on the risk of acute myeloid leukemia was analyzed using receiver operating curve(ROC).100 patients were also divided into the high miRNA-107 expression group(50 cases)and low miRNA-107 expression group(50 cases)according to the median expression level of miRNA-107.The relationships between miRNA-107 expression and clinical indexes,gene mutations and prognosis were analyzed in both groups.Results The level of miRNA-107 in the observation group was significantly lower than that of the control group(P<0.05),and the area under the ROC curve of miRNA-107 for predicting the risk of acute myeloid leukemia was 0.877.The HGB level,proportion of NPM1 wild type and complete remission rate in the high miRNA-107 expression group were significantly higher than those of the low miRNA-107 expression group(P<0.05).There were no significant differences in gender,age,WBC,PLT,bone marrow blast cell ratio,CEBPα,FLT3-ITD,IDH1/2 and DNMT3A between the two groups(P>0.05).The median overall survival(OS)time and median recurrence-free survival(RFS)time which were 26 months and 21.5 months in the high miRNA-107 expression group were significantly higher than those of the low miRNA-107 expression group which were 15 months and 11.8 months(P<0.05).Multivariate analysis using Cox regression model showed that low miRNA-107 expression was an independent risk factor for poor prognosis in patients with acute myeloid leukemia(P<0.05).Conclusion High expression of mi
作者
黄凤霞
李海燕
苏媛媛
黄君华
雷燕
HUANG Feng-xia;LI Hai-yan;SU Yuan-yuan;HUANG Jun-hua;LEI Yan(Department of Medical Technology Clinical and Hematological Laboratory,Xi′an Medical University,Xi′an 710021,China;Department of Clinical Laboratory,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)
出处
《标记免疫分析与临床》
CAS
2019年第12期1988-1993,共6页
Labeled Immunoassays and Clinical Medicine
基金
国家自然科学基金青年项目(编号:81700546)
西安医学院2018年校级大学生创新创业训练计划项目(编号:2018DC-91)
