摘要
细菌可存在于包括自来水和饮用水在内的所有水环境中,也可以生物膜的形式附着在固体表面。余氯的存在会使细胞膜和膜相关聚合物的化学官能团发生形变,阻止细菌由可逆附着向不可逆附着的转变,从而阻碍了生物膜在新的表面扩散和定植。本文以铜绿假单胞菌为例,运用激光共聚焦显微镜的成像技术,有效地表征了细菌生物膜在初期形成过程中的三维形态结构,并探究了消毒剂胁迫作用下生物膜形成的差异性,可为铜绿假单胞菌的病理性研究提供可视化数据支持,具有指导意义。
Bacteria could live in extracellular polysaccharides and be found in water environments including tap water and drinking water which tended to attach to solid surface. The presence of chlorine deformed the chemical functional groups of the cell membrane and membrane-associated polymers, prevented the transition from reversible attachment to irreversible attachment, thereby hindering the diffusion and colonization of biofilms on new surfaces. In this study, the imaging technique of laser confocal microscopy was used to effectively characterize the three-dimensional structure of biofilm during the initial formation process, and explore the difference of biofilm formation under the disinfectant disinfection which Pseudomonas aeruginosa was taken as a precedent. Research could provide visual data support, which was instructive to the study of pathological of Pseudomonas aeruginosa.
作者
杜邦
单蓉蓉
谷正
Du Bang;Shan Rong-rong;Gu Zheng(College of Civil and Water Engineering,Hefei University of Technology,Anhui Hefei 230009)
出处
《生物化工》
2019年第6期80-82,共3页
Biological Chemical Engineering
关键词
铜绿假单胞菌
生物膜
初期附着
可视化技术
Pseudomonas aeruginosa
Biofilm
Initial attachment
Visualization technology
