摘要
目的:探讨丹酚酸B(Sal B)对成骨细胞氧化损伤的保护作用。方法:构建叔丁基过氧化氢诱导成骨细胞氧化损伤模型,通过MTT实验检测细胞活性,DCFH-DA荧光染色检测细胞活性氧水平,qPCR检测相关成骨分化基因表达水平,并通过茜素红染色检测成骨细胞的矿化水平,从而评价Sal B对成骨细胞氧化损伤的保护作用。结果:MTT检测结果显示叔丁基过氧化氢对成骨细胞活性的抑制呈时间和浓度依赖性,250μmol/L浓度作用6 h达到半数抑制。Sal B的应用可恢复成骨细胞活性至84.6%,降低了活性氧水平(P<0.001),恢复了分化相关基因表达水平及细胞外基质矿化水平(P<0.001)。结论:Sal B可有效抑制成骨细胞的氧化损伤,恢复成骨细胞活性和功能。
Objective:To explore the protective effect of salvianolic acid B(Sal B)on oxidative damage of osteoblasts.Methods:The oxidative damage model of osteoblasts induced by tert-butyl hydroperoxide(TBHP)was constructed.Cell viability was detected by MTT assay.The level of reactive oxygen species(ROS)was detected by DCFH-DA fluorescence staining.Real-time PCR was used to assess the levels of osteogenic differentiation genes.Alizarin red staining was used to detect the extracellular matrix mineralization of osteoblasts.Results:The results of MTT showed that the inhibition of osteoblast viability by TBHP was in a time-and concentrationdependent manner.Treatment with 250μmol/L TBHP for 6 h was the half inhibitory condition for osteoblasts.Sal B significantly restored cell viability up to 84.6%,the expression of differentiation genes and extracellular matrix mineralization level of osteoblasts(P<0.001)and meanwhile decreased the level of ROS(P<0.001).Conclusion:Sal B effectively inhibited the oxidative damage and restored osteoblasts’viability and function.
作者
荣彩丽
桑春辉
陈东
唐琪
RONG Caili;SANG Chunhui;CHEN Dong;TANG Qi(Department of Stomatology,Ningbo Yinzhou No.2 Hospital,Ningbo 315000,China;Department of Endodontics,Hospital of Stomatology,Haishu District,Ningbo 315000,China;Department of Pathology,Ningbo Yinzhou No.2 Hospital,Ningbo 315000,China;Department of Stomatology,the Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310009,China)
出处
《温州医科大学学报》
CAS
2019年第12期867-871,877,共6页
Journal of Wenzhou Medical University
基金
浙江省自然科学基金资助项目（LY15H140003）
